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Immunofluorescent staining of human cell line A-431 shows localization to cytosol & centrosome.
Antibody dilution
1:53
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA017327
Antibody HPA035026
Standard validation
Approved
Figure description
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Expression
RNA: detected in 29 tissues Protein: detected in 54 cell types
Retrieval
HIER pH6
Antibody dilution
1:50
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA017327
Antibody HPA035026
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
No bands detected.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402302)
Target mass (kDa)
109, 105.3, 104.6, 102.1, 100.1
109, 105.3, 104.6, 100.1
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA017327
Antibody HPA035026
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
