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Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.
Immunohistochemical staining of human placenta shows strong positivity in endothelial cells.
Expression
RNA: detected in 36 tissues Protein: detected in 22 cell types
RNA: detected in 36 tissues Protein: detected in 16 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:60
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA010718
Antibody HPA014730
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402871)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
35, 34.9, 9.6
35, 34.9, 34, 33.4, 31.9
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA010718
Antibody HPA014730
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
