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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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MCL1
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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

MCL1

Gene description

Myeloid cell leukemia 1

Protein class

Cancer-related genes
Plasma proteins
Predicted intracellular proteins
Predicted membrane proteins

Predicted localization

Intracellular,Membrane

Number of transcripts

3
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Cell line enriched (U-698)
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Granular cytoplasmic expression in most tissues.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data. Caution, Splice and/or transcript discrepancy exists.

Reliability score

Approved based on 4 antibodies.
HPA008455 , HPA031125 , CAB002781 , CAB068195
SHOW MORE

PROGNOSTIC SUMMARY

Prognostic marker in renal cancer (unfavourable).
Renal cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Thyroid cancer

STAINING SUMMARY

HPA008455
HPA031125
CAB002781
CAB068195

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Strong cytoplasmic staining in most testis cancers. Weak to moderate staining in most other malignant tissues. Virtually no staining in malignant gliomas, skin and renal carcinomas.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Moderate cytoplasmic positivity was observed in most malignancies. Thyroid cancer and few malignant melanomas were strongly stained. Skin and several malignant gliomas, cervical, urothelial and renal cancers were negative or weakly stained.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most tumor cells showed moderate to strong nuclear positivity. Ovarian and endometrial cancers were mainly weak or negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most cancers showed weak to moderate cytoplasmic immunoreactivity. A few cases of lymphoma, breast, cervical and testis cancers displayed strong positivity. Several gliomas, protate, endometrial, lung, skin, renal and liver cancer were negative.

GENE INFORMATION

Gene name

MCL1 (HGNC Symbol)

Synonyms

BCL2L3, Mcl-1

Description

Myeloid cell leukemia 1 (HGNC Symbol)

Entrez gene summary

This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]

Chromosome

1

Cytoband

q21.2

Chromosome location (bp)

150574551 - 150579738

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000143384 (version 83.38)

Entrez gene

4170

UniProt

Q07820 (UniProt - Evidence at protein level)

neXtProt

NX_Q07820

Antibodypedia

MCL1 antibodies

PROTEIN BROWSER

MCL1-001
MCL1-002
MCL1-201

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

MCL1-001
ENSP00000358022
ENST00000369026
Q07820 [Direct mapping]
Induced myeloid leukemia cell differentiation protein Mcl-1
Show all
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001709 [cell fate determination]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005739 [mitochondrion]
GO:0005741 [mitochondrial outer membrane]
GO:0005759 [mitochondrial matrix]
GO:0005829 [cytosol]
GO:0007275 [multicellular organismal development]
GO:0008630 [intrinsic apoptotic signaling pathway in response to DNA damage]
GO:0008637 [apoptotic mitochondrial changes]
GO:0015266 [protein channel activity]
GO:0016020 [membrane]
GO:0016021 [integral component of membrane]
GO:0019725 [cellular homeostasis]
GO:0034097 [response to cytokine]
GO:0042803 [protein homodimerization activity]
GO:0042981 [regulation of apoptotic process]
GO:0043066 [negative regulation of apoptotic process]
GO:0046982 [protein heterodimerization activity]
GO:0051434 [BH3 domain binding]
GO:0071806 [protein transmembrane transport]
GO:0097136 [Bcl-2 family protein complex]
GO:0097192 [extrinsic apoptotic signaling pathway in absence of ligand]
GO:1903378 [positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway]
GO:2000811 [negative regulation of anoikis]
GO:2001020 [regulation of response to DNA damage stimulus]
GO:2001240 [negative regulation of extrinsic apoptotic signaling pathway in absence of ligand]
GO:2001243 [negative regulation of intrinsic apoptotic signaling pathway]
Show all
350 aa
37.3 kDa
No 1
MCL1-002
ENSP00000309973
ENST00000307940
Q07820 [Direct mapping]
Induced myeloid leukemia cell differentiation protein Mcl-1
Show all
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001709 [cell fate determination]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005739 [mitochondrion]
GO:0005741 [mitochondrial outer membrane]
GO:0005829 [cytosol]
GO:0007275 [multicellular organismal development]
GO:0008630 [intrinsic apoptotic signaling pathway in response to DNA damage]
GO:0015266 [protein channel activity]
GO:0016020 [membrane]
GO:0016021 [integral component of membrane]
GO:0019725 [cellular homeostasis]
GO:0034097 [response to cytokine]
GO:0042803 [protein homodimerization activity]
GO:0042981 [regulation of apoptotic process]
GO:0046982 [protein heterodimerization activity]
GO:0051434 [BH3 domain binding]
GO:0071806 [protein transmembrane transport]
GO:0097136 [Bcl-2 family protein complex]
GO:0097192 [extrinsic apoptotic signaling pathway in absence of ligand]
GO:1903378 [positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway]
GO:2000811 [negative regulation of anoikis]
GO:2001020 [regulation of response to DNA damage stimulus]
GO:2001240 [negative regulation of extrinsic apoptotic signaling pathway in absence of ligand]
GO:2001243 [negative regulation of intrinsic apoptotic signaling pathway]
Show all
271 aa
28.7 kDa
No 0
MCL1-201
ENSP00000477624
ENST00000620947
A0A087WT64 [Direct mapping]
Induced myeloid leukemia cell differentiation protein Mcl-1
Show all
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005622 [intracellular]
GO:0008630 [intrinsic apoptotic signaling pathway in response to DNA damage]
GO:0016021 [integral component of membrane]
GO:0042803 [protein homodimerization activity]
GO:0042981 [regulation of apoptotic process]
GO:2001243 [negative regulation of intrinsic apoptotic signaling pathway]
Show all
197 aa
21.2 kDa
No 1

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