EPHX1 - Antibodies - The Human Protein Atlas

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EPHX1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA020593

Antibody HPA048847

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA020593

HPA048847

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA020593, RRID:AB_10601373

Atlas Antibodies Cat#HPA048847, RRID:AB_2680538

VALIDATION SUMMARY

Antibody HPA020593

Antibody HPA048847

Immunohistochemistry

Western blot

Protein array

IMMUNOHISTOCHEMISTRY

Antibody HPA020593

Antibody HPA048847

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Expression

RNA: detected in 37 tissues
Protein: detected in 16 cell types

RNA: detected in 37 tissues
Protein: detected in 11 cell types

Retrieval

HIER pH6

Antibody dilution

1:3000

1:1000

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA020593

Antibody HPA048847

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

52.9, 19.3, 16.4

52.9

Antibody dilution

1:250

1:410

PROTEIN ARRAY

Antibody HPA020593

Antibody HPA048847

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA020593

Antibody HPA048847

Antigen

Recombinant protein fragment

Length (aa)

107

100

Antigen sequence

DKEETLPLEDGWWGPGTRSAAREDDSIRPFKVETSDEEIHDLHQRIDKFR
FTPPLEDSCFHYGFNSNYLKKVISYWRNEFDWKKQVEILNRHPHFKTKIE
GLDIHFI

LPAGHTPKPLLMVHGWPGSFYEFYKIIPLLTDPKNHGLSDEHVFEVICPS
IPGYGFSEASSKKGFNSVATARIFYKLMLRLGFQEFYIQGGDWGSLICTN

Matching transcripts

EPHX1-001 - ENSP00000355802 [99%]
EPHX1-002 - ENSP00000398491 [99%]
EPHX1-003 - ENSP00000408469 [99%]
EPHX1-201 - ENSP00000272167 [99%]
EPHX1-202 - ENSP00000480004 [99%]

EPHX1-001 - ENSP00000355802 [100%]
EPHX1-201 - ENSP00000272167 [100%]
EPHX1-202 - ENSP00000480004 [100%]

Other gene match

ANTIGEN VIEW

EPHX1-001	EPHX1-002	EPHX1-003	EPHX1-201	EPHX1-202

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.