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IGFBP1

ANTIBODY INFORMATION
 
 

Antibody HPA046972

  

Antibody HPA050640

  

Antibody CAB004445

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA046972   HPA050640   sc-25257  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 12   12   3  

References

 1      

Proper citation

 Atlas Antibodies Cat#HPA046972, RRID:AB_2679884   Atlas Antibodies Cat#HPA050640, RRID:AB_2681197   Santa Cruz Biotechnology Cat#sc-25257, RRID:AB_627782  

VALIDATION SUMMARY
 
 

Antibody HPA046972

  

Antibody HPA050640

  

Antibody CAB004445

  
Immunocytochemistry

Supported      
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot

Uncertain   Uncertain   Supported  
Protein array

Supported   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA046972

  

Antibody HPA050640

  

Antibody CAB004445

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.      

Antibody dilution

 1:40      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA046972

  

Antibody HPA050640

  

Antibody CAB004445

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA050640 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA046972 across 46 cell lines.		    
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA050640 across 76 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA046972 across 76 cell types.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in decidual cells.   Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in decidual cells.   Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in decidual cells.  

Expression

 RNA: detected in 7 tissues
Protein: detected in 2 cell types		   RNA: detected in 7 tissues
Protein: detected in 4 cell types		   RNA: detected in 7 tissues
Protein: detected in 3 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:3000   1:7000   1:10000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA046972

  

Antibody HPA050640

  

Antibody CAB004445

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 27.9, 27.7, 22.9   27.9, 27.7   27.9, 27.7, 22.9  

Antibody dilution

 1:200   1:360   1:500  

PROTEIN ARRAY
 
 

Antibody HPA046972

  

Antibody HPA050640

  

Antibody CAB004445

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1600   1:2850    

ANTIGEN INFORMATION
 
 

Antibody HPA046972

  

Antibody HPA050640

  

Antibody CAB004445

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 71   93    

Antigen sequence

 
QQPLHALTRGQGACVQESDASAPHAAEAGSPESPESTEITEEELLDNFHL
MAPSEEDHSILWDAISTYDGS
  
SKALHVTNIKKWKEPCRIELYRVVESLAKAQETSGEEISKFYLPNCNKNG
FYHSRQCETSMDGEAGLCWCVYPWNGKRIPGSPEIRGDPNCQI
  

											 
																				

																		

										
											
Matching transcripts

												
																					IGFBP1-001 - ENSP00000275525 [100%] 
IGFBP1-002 - ENSP00000413511 [100%] 
IGFBP1-003 - ENSP00000417069 [100%] 

											 
																						IGFBP1-001 - ENSP00000275525 [100%] 
IGFBP1-002 - ENSP00000413511 [98%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
IGFBP1-001
IGFBP1-002
IGFBP1-003
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA046972

												
										 
																				
											
											
											
Antibody HPA050640

												
										 
																				
											
											
											
Antibody CAB004445

												
										 
																		

																	

										
											
1.

												
																						Affinity proteomics reveals elevated muscle proteins in plasma of children with cerebral malaria

	Bachmann J et al

	PLoS Pathog 2014;10(4):e1004038

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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