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ANTIBODY INFORMATION
 
 

Antibody HPA000263

  

Antibody HPA011135

  

Antibody HPA011227

  

Antibody CAB010898

  

Antibody CAB047336

  

Antibody CAB047338

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   SIGMA
   NCI-CPTC
   NCI-CPTC
  

Product name

 HPA000263   HPA011135   HPA011227   M7060   FSAI004:9E7   CPTC-MSN-3  

Host species

 Rabbit   Rabbit   Rabbit   Mouse   Mouse   Mouse  

Clonality

 pAb   pAb   pAb   mAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known   Protein A/G   Protein A/G  

Other gene match

 RDX - ENSG00000137710 [100%]
            

Released in version

 4   4   4   4   12   9  

References

            

Proper citation

 Atlas Antibodies Cat#HPA000263, RRID:AB_1856043   Atlas Antibodies Cat#HPA011135, RRID:AB_1854053   Atlas Antibodies Cat#HPA011227, RRID:AB_1854054   Sigma-Aldrich Cat#M7060, RRID:AB_260635      

VALIDATION SUMMARY
 
 

Antibody HPA000263

  

Antibody HPA011135

  

Antibody HPA011227

  

Antibody CAB010898

  

Antibody CAB047336

  

Antibody CAB047338

  
Immunocytochemistry

 

Validated
     Supported   Supported   Supported  
Immunohistochemistry

Supported   Supported   Supported  

Validated
    

Validated
  
Western blot

Supported  

Validated
   Supported   Uncertain   Uncertain   Supported  
Protein array

Approved   Supported   Approved        

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000263

  

Antibody HPA011135

  

Antibody HPA011227

  

Antibody CAB010898

  

Antibody CAB047336

  

Antibody CAB047338

  

Formal validation: Genetic

  
Validated

Significant downregulation > 25% by one siRNA.		          

 
         

Figure description

   Cytoplasmic region of segmented cells in 10x-images          

Antibody dilution

   1:55          
 

Standard validation

   Supported     Supported   Supported   Supported  

           

Figure description

   Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.     Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.   Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.   Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.  

Antibody dilution

   1:54     1:2000   1:2000   1:2000  

Literature conformity

   The subcellular location is supported by literature.     The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000263

  

Antibody HPA011135

  

Antibody HPA011227

  

Antibody CAB010898

  

Antibody CAB047336

  

Antibody CAB047338

  

Formal validation: Orthogonal

      
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

     
   
 

Figure description

       Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.  
 

Standard validation

 Supported   Supported   Supported   Supported     Supported  

           

Figure description

 Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human tonsil shows positivity in the germinal centre.   Immunohistochemical staining of human tonsil shows positivity in the germinal centre.   Immunohistochemical staining of human tonsil shows positivity in the germinal centre.     Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in non-germinal center cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 40 cell types		   RNA: detected in 37 tissues
Protein: detected in 29 cell types		   RNA: detected in 37 tissues
Protein: detected in 63 cell types		   RNA: detected in 37 tissues
Protein: detected in 38 cell types		     RNA: detected in 37 tissues
Protein: detected in 52 cell types		  

Retrieval

 HIER pH9   HIER pH6   HIER pH6   HIER pH6     HIER pH6  

Antibody dilution

 1:150   1:750   1:75   1:80000     1:60000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.     Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA000263

  

Antibody HPA011135

  

Antibody HPA011227

  

Antibody CAB010898

  

Antibody CAB047336

  

Antibody CAB047338

  

Formal validation: Genetic

  
Validated

Downregulation visible in one of two siRNA lanes		          

           

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		          

Target mass (kDa)

   67.8          

Loading control

   Total protein image          

Antibody dilution

   1:119          
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

           

Figure description

 Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 71, 68.6, 67.8, 23.4, 17.3   67.8   67.8   67.8, 12.1   67.8, 12.1   67.8, 12.1  

Antibody dilution

 1:500   1:250   1:250   1:500   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA000263

  

Antibody HPA011135

  

Antibody HPA011227

  

Antibody CAB010898

  

Antibody CAB047336

  

Antibody CAB047338

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		        

           

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.        

Antibody dilution

 1:2000   1:3000   1:500        

ANTIGEN INFORMATION
 
 

Antibody HPA000263

  

Antibody HPA011135

  

Antibody HPA011227

  

Antibody CAB010898

  

Antibody CAB047336

  

Antibody CAB047338

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Not known   Recombinant protein   Recombinant protein  

Length (aa)

 122   101   110        

Antigen sequence

 
SKGYSTWLKLNKKVTQQDVKKENPLQFKFRAKFFPEDVSEELIQEITQRL
FFLQVKEAILNDEIYCPPETAVLLASYAVQAKYGDYNKEIHKPGYLANDR
LLPQRVLEQHKLTKEQWEERIQ
  
KKAQQELEEQTRRALELEQERKRAQSEAEKLAKERQEAEEAKEALLQASR
DQKKTQEQLALEMAELTARISQLEMARQKKESEAVEWQQKAQMVQEDLEK
T
  
AELKTAMSTPHVAEPAENEQDEQDENGAEASADLRADAMAKDRSEEERTT
EAEKNERVQKHLKALTSELANARDESKKTANDMIHAENMRLGRDKYKTLR
QIRQGNTKQR
  

											 
																						

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					MSN-001 - ENSP00000353408 [85%] 

											 
																						MSN-001 - ENSP00000353408 [100%] 

											 
																						MSN-001 - ENSP00000353408 [100%] 

											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					RDX - ENSG00000137710 [100%]

											 
																						
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
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