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Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblasts.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblasic cells.
Expression
RNA: detected in 3 tissues Protein: detected in 71 cell types
RNA: detected in 3 tissues Protein: detected in 71 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:65
1:2500
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
Standard validation
Supported
Figure description
Neuronal sonata and numerous fiber bundles stained throughout the brain.
WESTERN BLOT
Antibody HPA046846
Antibody HPA062111
Antibody CAB010885
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
21.4
21.4
21.4
Antibody dilution
1:500
1:470
1:500
PROTEIN ARRAY
Antibody HPA046846
Antibody HPA062111
Antibody CAB010885
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
