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HERC4

ANTIBODY INFORMATION
 
 

Antibody HPA045453

  

Antibody HPA070301

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA045453   HPA070301  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 9   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA045453, RRID:AB_10961917   Atlas Antibodies Cat#HPA070301, RRID:AB_2686247  

VALIDATION SUMMARY
 
 

Antibody HPA045453

  

Antibody HPA070301

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Uncertain    
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA045453

  

Antibody HPA070301

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to nucleoli fibrillar center & cytosol.  

Antibody dilution

   1:73  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA045453

  

Antibody HPA070301

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in subset of glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 4 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:75    

Literature conformity

 Partly consistent with gene/protein characterization data.    

RNA consistency

 Not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA045453

  

Antibody HPA070301

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 118.6, 117.7, 109.6, 106.7   118.6, 117.7, 109.6, 25.9, 12  

Antibody dilution

 1:100   1:240  

PROTEIN ARRAY
 
 

Antibody HPA045453

  

Antibody HPA070301

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:850   1:2900  

ANTIGEN INFORMATION
 
 

Antibody HPA045453

  

Antibody HPA070301

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 92   74  

Antigen sequence

 
FHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLM
SDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLK
  
GNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGT
VYTCGCNDLGQLGHEKSRKKPEQV
 

Matching transcripts

 HERC4-001 - ENSP00000362804 [100%]
HERC4-002 - ENSP00000277817 [100%]
HERC4-008 - ENSP00000416504 [100%]
HERC4-009 - ENSP00000378624 [100%]
  HERC4-001 - ENSP00000362804 [100%]
HERC4-008 - ENSP00000416504 [100%]
HERC4-009 - ENSP00000378624 [100%]
HERC4-015 - ENSP00000427191 [100%]
HERC4-006 - ENSP00000422383 [97%]
 

Other gene match

    
ANTIGEN VIEW
HERC4-001
HERC4-002
HERC4-006
HERC4-008
HERC4-009
HERC4-015
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.