CHEK1 - Antibodies - The Human Protein Atlas

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CHEK1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA044364

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA044364

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA044364, RRID:AB_2678913

VALIDATION SUMMARY

Antibody HPA044364

Immunocytochemistry

Supported

Western blot

Uncertain

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA044364

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.

Antibody dilution

1:61

Literature conformity

The subcellular location is supported by literature.

WESTERN BLOT

Antibody HPA044364

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Target mass (kDa)

55.5, 54.4, 50.4, 49, 43.7

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA044364

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA044364

Antigen

Recombinant protein fragment

Length (aa)

130

Antigen sequence

KRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLN
PWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVT
SGGVSESPSGFSKHIQSNLDFSPVNSASSE

Matching transcripts

CHEK1-001 - ENSP00000391090 [100%]
CHEK1-002 - ENSP00000412504 [100%]
CHEK1-004 - ENSP00000435371 [100%]
CHEK1-005 - ENSP00000442317 [100%]
CHEK1-006 - ENSP00000278916 [100%]
CHEK1-007 - ENSP00000432890 [100%]
CHEK1-201 - ENSP00000388648 [100%]
CHEK1-202 - ENSP00000481616 [100%]

Other gene match

ANTIGEN VIEW

CHEK1-001	CHEK1-002	CHEK1-004	CHEK1-005	CHEK1-006	CHEK1-007	CHEK1-201	CHEK1-202

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.