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THYN1

ANTIBODY INFORMATION
 
 

Antibody HPA038732

  

Antibody HPA038733

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA038732   HPA038733  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   12  

References

    

Proper citation

 Atlas Antibodies Cat#HPA038732, RRID:AB_10697397   Atlas Antibodies Cat#HPA038733, RRID:AB_2676183  

VALIDATION SUMMARY
 
 

Antibody HPA038732

  

Antibody HPA038733

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA038732

  

Antibody HPA038733

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli fibrillar center.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus, nucleoli fibrillar center & cytosol.  

Antibody dilution

 1:61   1:39  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA038732

  

Antibody HPA038733

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.   Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 81 cell types		   RNA: detected in 37 tissues
Protein: detected in 78 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:75  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA038732

  

Antibody HPA038733

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 25.7   25.7, 18.8  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA038732

  

Antibody HPA038733

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA038732

  

Antibody HPA038733

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 82   96  

Antigen sequence

 
AFFYHSNCKEPGIAGLMKIVKEAYPDHTQFEKNNPHYDPSSKEDNPKWSM
VDVQFVRMMKRFIPLAELKSYHQAHKATGGPL
  
KGLSGKRTKTENSGEALAKVEDSNPQKTSATKNCLKNLSSHWLMKSEPES
RLEKGVDVKFSIEDLKAQPKQTTCWDGVRNYQARNFLRAMKLGEEA
 

Matching transcripts

 THYN1-002 - ENSP00000341657 [100%]
THYN1-004 - ENSP00000376373 [100%]
THYN1-005 - ENSP00000376374 [100%]
  THYN1-001 - ENSP00000341452 [100%]
THYN1-002 - ENSP00000341657 [100%]
THYN1-004 - ENSP00000376373 [100%]
THYN1-005 - ENSP00000376374 [100%]
 

Other gene match

    
ANTIGEN VIEW
THYN1-001
THYN1-002
THYN1-004
THYN1-005
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.