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Antibody staining overlaps with antibody HPA051575.
Validated
Antibody staining overlaps with antibody HPA010738.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Antibody dilution
1:15
1:66
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA010738
Antibody HPA051575
Antibody CAB025906
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human small intestine shows membranous and cytoplasmic positivity.
Immunohistochemical staining of human stomach, upper shows cytoplasmic and nuclear positivity.
Expression
RNA: detected in 37 tissues Protein: detected in 73 cell types
RNA: detected in 37 tissues Protein: detected in 70 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:15
1:75
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA010738
Antibody HPA051575
Antibody CAB025906
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
93, 33.2, 30
93
93, 33.2, 30
Antibody dilution
1:250
1:80
1:500
PROTEIN ARRAY
Antibody HPA010738
Antibody HPA051575
Antibody CAB025906
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
