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XRCC4
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    TISSUE

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    CELL

  • PATHOLOGY

ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody HPA006801

 

Antibody HPA051538

 

Provider

Atlas Antibodies
Sigma-Aldrich
  Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA006801   HPA051538  

Host species

Rabbit   Rabbit  

Clonality

pAb   pAb  

Purity

Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

   

Released in version

4   12  

References

2    

Proper citation

Atlas Antibodies Cat#HPA006801, RRID:AB_1858899   Atlas Antibodies Cat#HPA051538, RRID:AB_2681526  

VALIDATION SUMMARY

 
 

Antibody HPA006801

 

Antibody HPA051538

 
Immunocytochemistry


Validated
 

Validated
 
Immunohistochemistry

Supported    
Western blot


Validated
  Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY

 
 

Antibody HPA006801

 

Antibody HPA051538

 

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA051538.
 
Validated

Antibody staining overlaps with antibody HPA006801.
 
 

Standard validation

Supported   Supported  

   

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.  

Antibody dilution

1:65   1:24  

Literature conformity

The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY

 
 

Antibody HPA006801

 

Antibody HPA051538

 

Standard validation

Supported    

   

Figure description

Immunohistochemical staining of human lymph node shows strong nuclear positivity in germinal center cells,.    

Expression

RNA: detected in 35 tissues
Protein: detected in 72 cell types
   

Retrieval

HIER pH6    

Antibody dilution

1:250    

Literature conformity

Consistent with extensive gene/protein characterization data.    

RNA consistency

Mainly consistent with RNA expression data.    

WESTERN BLOT

 
 

Antibody HPA006801

 

Antibody HPA051538

 

Formal validation: Genetic

Validated

Downregulation visible in both siRNA lanes
   

   

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled
   

Target mass (kDa)

38.3, 38.1    

Loading control

Total protein image    

Antibody dilution

1:142    
 

Standard validation

Supported

Band of predicted size in kDa (+/-20%) with additional bands present.
  Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.
 

   

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402930)
   

Target mass (kDa)

38.3, 38.1   38.3, 38.1  

Antibody dilution

1:250   1:120  

PROTEIN ARRAY

 
 

Antibody HPA006801

 

Antibody HPA051538

 

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.
  Supported

Pass with single peak corresponding to interaction only with its own antigen.
 

   

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

1:3000   1:950  

ANTIGEN INFORMATION

 
 

Antibody HPA006801

 

Antibody HPA051538

 

Antigen

Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

133   133  

Antigen sequence

KCVSAKEALETDLYKRFILVLNEKKTKIRSLHNKLLNAAQEREKDIKQEG
ETAICSEMTADRDPVYDESTDEESENQTDLSGLASAAVSKDDSIISSLDV
TDIAPSRKRRQRMQRNLGTEPKMAPQENQLQEK
 
IHLVSEPSITHFLQVSWEKTLESGFVITLTDGHSAWTGTVSESEISQEAD
DMAMEKGKYVGELRKALLSGAGPADVYTFNFSKESCYFFFEKNLKDVSFR
LGSFNLEKVENPAEVIRELICYCLDTIAENQAK
 

Matching transcripts

XRCC4-001 - ENSP00000379344 [100%]
XRCC4-002 - ENSP00000342011 [100%]
XRCC4-003 - ENSP00000421491 [100%]
XRCC4-201 - ENSP00000282268 [100%]
  XRCC4-001 - ENSP00000379344 [100%]
XRCC4-002 - ENSP00000342011 [100%]
XRCC4-003 - ENSP00000421491 [100%]
XRCC4-201 - ENSP00000282268 [100%]
 

Other gene match

   
ANTIGEN VIEW
XRCC4-001
XRCC4-002
XRCC4-003
XRCC4-201

RELEVANT PUBLICATIONS

 
 

Antibody HPA006801

 

Antibody HPA051538

 

1.

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23
   

2.

Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair
Reid DA et al
Proc Natl Acad Sci U S A 2015;112(20):E2575-84
Application: ICC-IF, WB
   
 

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