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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli fibrillar center.
Antibody dilution
1:10
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA011753
Antibody HPA017206
Antibody CAB004347
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubule.
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in the granular layer while Purkinje cells were moderately stained in the cytoplasm.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Expression
RNA: detected in 21 tissues Protein: detected in 45 cell types
RNA: detected in 21 tissues Protein: detected in 43 cell types
RNA: detected in 21 tissues Protein: detected in 41 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:175
1:25
1:10
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA011753
Antibody HPA017206
Antibody CAB004347
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
51.9
51.9
51.9, 15.5
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA011753
Antibody HPA017206
Antibody CAB004347
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
