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ANTIBODY INFORMATION
 
 

Antibody HPA048761

  

Antibody HPA058627

  

Antibody CAB020709

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Origene
  

Product name

 HPA048761   HPA058627   3127.00.02  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 12   14   5  

References

      

Proper citation

 Atlas Antibodies Cat#HPA048761, RRID:AB_2680514   Atlas Antibodies Cat#HPA058627, RRID:AB_2683782    

VALIDATION SUMMARY
 
 

Antibody HPA048761

  

Antibody HPA058627

  

Antibody CAB020709

  
Immunocytochemistry

Supported      
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA048761

  

Antibody HPA058627

  

Antibody CAB020709

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.      

Antibody dilution

 1:94      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA048761

  

Antibody HPA058627

  

Antibody CAB020709

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells in seminiferus ducts.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells in seminiferous ducts.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.  

Expression

 RNA: detected in 16 tissues
Protein: detected in 58 cell types		   RNA: detected in 16 tissues
Protein: detected in 1 cell types		   RNA: detected in 16 tissues
Protein: detected in 36 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:1800   1:150   1:1200  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA048761

  

Antibody HPA058627

  

Antibody CAB020709

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 70   70   70, 20.5  

Antibody dilution

 1:470   1:80   1:500  

PROTEIN ARRAY
 
 

Antibody HPA048761

  

Antibody HPA058627

  

Antibody CAB020709

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3750   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA048761

  

Antibody HPA058627

  

Antibody CAB020709

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Genetic immunization  

Length (aa)

 53   35    

Antigen sequence

 
ESEPEEKSEEEIEIIEGQEESNQSNKSGSEDEMKEADESTGSGDGPIKSV
RKR
  
QTKGVLEQEEKEEKAALEKPMDLEEEKKQNDGEML
  

											 
																				

																		

										
											
Matching transcripts

												
																					CLGN-001 - ENSP00000326699 [100%] 
CLGN-201 - ENSP00000392782 [100%] 

											 
																						CLGN-001 - ENSP00000326699 [100%] 
CLGN-201 - ENSP00000392782 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CLGN-001
CLGN-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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