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CD8A

ANTIBODY INFORMATION
 
 

Antibody HPA037756

  

Antibody CAB000012

  

Antibody CAB075722

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   DakoCytomation
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA037756   M7103   AMAb90883  

Host species

 Rabbit   Mouse   Mouse  

Clonality

 pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Supernatant   Protein A/G  

Other gene match

      

Released in version

 12   1   14  

References

 1      

Proper citation

 Atlas Antibodies Cat#HPA037756, RRID:AB_2675648   Dako Cat#M7103, RRID:AB_2075537   Atlas Antibodies Cat#AMAb90883, RRID:AB_2665709  

VALIDATION SUMMARY
 
 

Antibody HPA037756

  

Antibody CAB000012

  

Antibody CAB075722

  
Immunohistochemistry

Supported  

Validated
   Supported  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Approved      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA037756

  

Antibody CAB000012

  

Antibody CAB075722

  

Formal validation: Orthogonal

  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

 
   

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of non germinal center cells.   Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in lymphoid cells outside reaction centra.   Immunohistochemical staining of human duodenum shows cytoplasmic positivity in subsets of immune cells  

Expression

 RNA: detected in 35 tissues
Protein: detected in 15 cell types		   RNA: detected in 35 tissues
Protein: detected in 9 cell types		   RNA: detected in 35 tissues
Protein: detected in 5 cell types		  

Retrieval

 HIER pH6   HIER pH9   HIER pH6  

Antibody dilution

 1:800   1:100   1:250  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA037756

  

Antibody CAB000012

  

Antibody CAB075722

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 25.7, 22.1, 21.6   25.7, 22.1, 21.6   25.7, 22.1, 21.6  

Antibody dilution

 1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA037756

  

Antibody CAB000012

  

Antibody CAB075722

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:12000      

ANTIGEN INFORMATION
 
 

Antibody HPA037756

  

Antibody CAB000012

  

Antibody CAB075722

  

Antigen

 Recombinant protein fragment   Synthetic peptide   Recombinant protein  

Length (aa)

 84      

Antigen sequence

 
AASPTFLLYLSQNKPKAAEGLDTQRFSGKRLGDTFVLTLSDFRRENEGYY
FCSALSNSIMYFSHFVPVFLPAKPTTTPAPRPPT
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					CD8A-001 - ENSP00000283635 [100%] 
CD8A-002 - ENSP00000321631 [100%] 
CD8A-006 - ENSP00000386559 [100%] 
CD8A-004 - ENSP00000387314 [85%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CD8A-001
CD8A-002
CD8A-004
CD8A-006
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA037756

												
										 
																				
											
											
											
Antibody CAB000012

												
										 
																				
											
											
											
Antibody CAB075722

												
										 
																		

																	

										
											
1.

												
																						The transcriptomic and proteomic landscapes of bone marrow and secondary lymphoid tissues

	Andersson S et al

	PLoS One 2014;9(12):e115911

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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