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Antibody staining overlaps with antibody HPA059332.
Validated
Antibody staining overlaps with antibody HPA037673.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & nuclear speckles.
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & nuclear speckles.
Antibody dilution
1:98
1:200
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA037673
Antibody HPA037674
Antibody HPA059332
Standard validation
Supported
Figure description
Immunohistochemical staining of human pancreas shows moderate nuclear and cytoplasmic positivity in exocrine glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 56 cell types
Retrieval
HIER pH6
Antibody dilution
1:75
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA037673
Antibody HPA037674
Antibody HPA059332
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
71.6, 59.4, 7.8
71.6, 59.4
71.6, 59.4
Antibody dilution
1:250
1:250
1:680
PROTEIN ARRAY
Antibody HPA037673
Antibody HPA037674
Antibody HPA059332
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
