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ADAMTS5

ANTIBODY INFORMATION
 
 

Antibody HPA005661

  

Antibody HPA030906

  

Antibody HPA030908

  

Antibody CAB025996

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA005661   HPA030906   HPA030908   MAB2198  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

        

Released in version

 3   14   14   6  

References

        

Proper citation

 Atlas Antibodies Cat#HPA005661, RRID:AB_1078101   Atlas Antibodies Cat#HPA030906, RRID:AB_2673654   Atlas Antibodies Cat#HPA030908, RRID:AB_2673655    

VALIDATION SUMMARY
 
 

Antibody HPA005661

  

Antibody HPA030906

  

Antibody HPA030908

  

Antibody CAB025996

  
Immunocytochemistry

  Approved   Approved    
Immunohistochemistry

Approved       Approved  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA005661

  

Antibody HPA030906

  

Antibody HPA030908

  

Antibody CAB025996

  

Standard validation

   Approved   Approved    

       

Figure description

   Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.    

Antibody dilution

   1:27   1:21    

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA005661

  

Antibody HPA030906

  

Antibody HPA030908

  

Antibody CAB025996

  

Standard validation

 Approved       Approved  

       

Figure description

 Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in glial cells.       Immunohistochemical staining of human fallopian tube shows strong positivity in cytoplasm and cilia of glandular cells.  

Expression

 RNA: detected in 25 tissues
Protein: detected in 58 cell types		       RNA: detected in 25 tissues
Protein: detected in 46 cell types		  

Retrieval

 HIER pH6       HIER pH6  

Antibody dilution

 1:25       1:80  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.       Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.       Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA005661

  

Antibody HPA030906

  

Antibody HPA030908

  

Antibody CAB025996

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

       

Figure description

 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 101.7   101.7   101.7   101.7  

Antibody dilution

 1:250   1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA005661

  

Antibody HPA030906

  

Antibody HPA030908

  

Antibody CAB025996

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA005661

  

Antibody HPA030906

  

Antibody HPA030908

  

Antibody CAB025996

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 116   77   78    

Antigen sequence

 
KGLVQNIDQLYSGGGKVGYLVYAGGRRFLLDLERDGSVGIAGFVPAGGGT
SAPWRHRSHCFYRGTVDGSPRSLAVFDLCGGLDGFFAVKHARYTLKPLLR
GPWAEEEKGRVYGDGS
  
KSTPKVNSVTSHGSNKVGSHTSQPQWVTGPWLACSRTCDTGWHTRTVQCQ
DGNRKLAKGCPLSQRPSAFKQCLLKKC
  
SHDDSKFCEETFGSTEDKRLMSSILTSIDASKPWSKCTSATITEFLDDGH
GNCLLDLPRKQILGPEELPGQTYDATQQ
  

											 
																				

																		

										
											
Matching transcripts

												
																					ADAMTS5-001 - ENSP00000284987 [100%] 

											 
																						ADAMTS5-001 - ENSP00000284987 [100%] 

											 
																						ADAMTS5-001 - ENSP00000284987 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

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