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OTUD6B

ANTIBODY INFORMATION
 
 

Antibody HPA024046

  

Antibody HPA024503

  

Antibody HPA024772

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA024046   HPA024503   HPA024772  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 6   5   5  

References

      

Proper citation

 Atlas Antibodies Cat#HPA024046, RRID:AB_10600925   Atlas Antibodies Cat#HPA024503, RRID:AB_1854845   Atlas Antibodies Cat#HPA024772, RRID:AB_1854846  

VALIDATION SUMMARY
 
 

Antibody HPA024046

  

Antibody HPA024503

  

Antibody HPA024772

  
Immunocytochemistry

Approved   Approved    
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA024046

  

Antibody HPA024503

  

Antibody HPA024772

  

Standard validation

 Approved   Approved    

     

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to cytosol & the Golgi apparatus.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.    

Antibody dilution

 1:20   1:50    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA024046

  

Antibody HPA024503

  

Antibody HPA024772

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules and glomeruli.   Immunohistochemical staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.   Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 77 cell types		   RNA: detected in 36 tissues
Protein: detected in 69 cell types		   RNA: detected in 36 tissues
Protein: detected in 75 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:125   1:350  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  
 

Standard validation

 Approved      

     

Figure description

 Subsets of astrocytes show distinct staining in many areas of the brain.      

WESTERN BLOT
 
 

Antibody HPA024046

  

Antibody HPA024503

  

Antibody HPA024772

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 37.3, 33.8   37.3, 33.8, 21.8   37.3, 33.8  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA024046

  

Antibody HPA024503

  

Antibody HPA024772

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA024046

  

Antibody HPA024503

  

Antibody HPA024772

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 61   67   64  

Antigen sequence

 
IQGMKNAVPKNDKKRRKQLTEDVAKLEKEMEQKHREELEQLKLTTKENKI
DSVAVNISNLV
  
HCMYKAIEDQLKEKDCALTVVALRSQTAEYMQSHVEDFLPFLTNPNTGDM
YTPEEFQKYCEDIVNTA
  
ENQPPRISKAQKRREKKAALEKEREERIAEAEIENLTGARHMESEKLAQI
LAARQLEIKQIPSD
 

Matching transcripts

 OTUD6B-001 - ENSP00000285420 [100%]
OTUD6B-004 - ENSP00000384190 [100%]
OTUD6B-202 - ENSP00000483706 [100%]
  OTUD6B-001 - ENSP00000285420 [100%]
OTUD6B-004 - ENSP00000384190 [100%]
OTUD6B-201 - ENSP00000481196 [100%]
OTUD6B-202 - ENSP00000483706 [100%]
  OTUD6B-001 - ENSP00000285420 [100%]
OTUD6B-004 - ENSP00000384190 [100%]
OTUD6B-202 - ENSP00000483706 [100%]
 

Other gene match

      
ANTIGEN VIEW
OTUD6B-001
OTUD6B-004
OTUD6B-201
OTUD6B-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.