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ANTIBODY INFORMATION
 
 

Antibody HPA059609

  

Antibody HPA062773

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA059609   HPA062773  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 13   13  

References

    

Proper citation

 Atlas Antibodies Cat#HPA059609, RRID:AB_2684082   Atlas Antibodies Cat#HPA062773, RRID:AB_2684865  

VALIDATION SUMMARY
 
 

Antibody HPA059609

  

Antibody HPA062773

  
Immunocytochemistry

Supported    
Immunohistochemistry

  Approved  
Western blot

Uncertain   Supported  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA059609

  

Antibody HPA062773

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to nucleus.    

Antibody dilution

 1:21    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA059609

  

Antibody HPA062773

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human lymph node shows strong cytoplasmic and nuclear positivity in germinal center cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 30 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:200  

Literature conformity

   Consistent with gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA059609

  

Antibody HPA062773

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 36.8, 30.5   36.8, 30.5, 22.9  

Antibody dilution

 1:80   1:150  

PROTEIN ARRAY
 
 

Antibody HPA059609

  

Antibody HPA062773

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:550   1:1850  

ANTIGEN INFORMATION
 
 

Antibody HPA059609

  

Antibody HPA062773

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 85   81  

Antigen sequence

 
FASNLYESECMNETEWTIYQDWHDWMNNQFGQSLELDGIIYLQATPETCL
HRIYLRGRNEEQGIPLEYLEKLHYKHESWLLHRTL
  
VNILKQLCEDWEVVPEPVARWCNVQSTQDEFEELTMSQKNGGNVLQMMYE
KPERWSFTFQTYACLSRIRAQLASLNGKLKD
 

Matching transcripts

 DCK-001 - ENSP00000286648 [100%]
DCK-005 - ENSP00000421508 [100%]
  DCK-001 - ENSP00000286648 [100%]
DCK-004 - ENSP00000425578 [100%]
DCK-005 - ENSP00000421508 [100%]
 

Other gene match

    
ANTIGEN VIEW
DCK-001
DCK-004
DCK-005
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.