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Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in plasma membrane & nucleus but excluded from the nucleoli and GFP expression in nucleus but excluded from the nucleoli.
Antibody dilution
1:35
Standard validation
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Antibody dilution
1:35
Literature conformity
The subcellular location is supported by literature.
WESTERN BLOT
Antibody HPA003176
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Target mass (kDa)
53, 30.4, 19.1
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA003176
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
