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SPPL3

ANTIBODY INFORMATION
 
 

Antibody HPA004194

  

Antibody HPA059958

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA004194   HPA059958  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 14   12  

References

    

Proper citation

 Atlas Antibodies Cat#HPA004194, RRID:AB_2667171   Atlas Antibodies Cat#HPA059958, RRID:AB_2684179  

VALIDATION SUMMARY
 
 

Antibody HPA004194

  

Antibody HPA059958

  
Immunocytochemistry

Supported    
Immunohistochemistry

  Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA004194

  

Antibody HPA059958

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & vesicles.    

Antibody dilution

 1:1000    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA004194

  

Antibody HPA059958

  

Standard validation

   Uncertain  

   

Figure description

   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells in seminiferous ducts.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 22 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:20  

Literature conformity

   Partly consistent with gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA004194

  

Antibody HPA059958

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 42.3   42.3  

Antibody dilution

 1:250   1:80  

PROTEIN ARRAY
 
 

Antibody HPA004194

  

Antibody HPA059958

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA004194

  

Antibody HPA059958

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 51   33  

Antigen sequence

 
FNSNVMVKVATQPADNPLDVLSRKLHLGPNVGRDVPRLSLPGKLVFPSST
G
  
VLRYDNYKKQASGDSCGAPGPANISGRMQKVSY
 

Matching transcripts

 SPPL3-001 - ENSP00000288680 [100%]
  SPPL3-001 - ENSP00000288680 [100%]
 

Other gene match

    
ANTIGEN VIEW
SPPL3-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.