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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Antibody dilution
1:57
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA006403
Standard validation
Approved
Figure description
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.
Expression
RNA: detected in 34 tissues Protein: detected in 75 cell types
Retrieval
HIER pH6
Antibody dilution
1:300
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Standard validation
Approved
Figure description
Cytoplasmic immunoreactivity in subsets of neurons as well as in many dendritic processes and synapses. Most abundant staining in areas of forebrain (olfactory and cortical areas predominantly) and some parts of hindbrain.
WESTERN BLOT
Antibody HPA006403
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
26.4, 24.3, 22.4
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA006403
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
