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CUL4B

ANTIBODY INFORMATION
 
 

Antibody HPA003046

  

Antibody HPA011880

  

Antibody HPA058979

  

Antibody CAB017786

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Origene
  

Product name

 HPA003046   HPA011880   HPA058979   2515.00.02  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

     CUL4A - ENSG00000139842 [100%]
    

Released in version

 2   4   14   4  

References

        

Proper citation

   Atlas Antibodies Cat#HPA011880, RRID:AB_1847340   Atlas Antibodies Cat#HPA058979, RRID:AB_2683868    

VALIDATION SUMMARY
 
 

Antibody HPA003046

  

Antibody HPA011880

  

Antibody HPA058979

  

Antibody CAB017786

  
Immunocytochemistry


Validated
  

Validated
   Approved    
Immunohistochemistry

  Supported     Supported  
Western blot

Uncertain   Supported   Uncertain   Supported  
Protein array

Supported   Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003046

  

Antibody HPA011880

  

Antibody HPA058979

  

Antibody CAB017786

  

Formal validation: Tagged protein
Validated

Antibody staining overlaps with GFP tagged protein		  
Validated

Antibody staining overlaps with GFP tagged protein		      

       

Figure description

 Immunofluorescent staining of transgenic HeLa cells show antibody staining in nucleoplasm and GFP expression in nucleoplasm.   Immunofluorescent staining of transgenic HeLa cells show antibody staining in nucleoplasm and GFP expression in nucleoplasm.      

Antibody dilution

 1:68   1:65      
 

Standard validation

 Supported   Supported   Approved    

       

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.    

Antibody dilution

 1:69   1:65   1:23    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003046

  

Antibody HPA011880

  

Antibody HPA058979

  

Antibody CAB017786

  

Standard validation

   Supported     Supported  

       

Figure description

   Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.     Immunohistochemical staining of human esophagus shows nuclear positivity in squamous epithelial cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 51 cell types		     RNA: detected in 37 tissues
Protein: detected in 69 cell types		  

Retrieval

   HIER pH6     HIER pH6  

Antibody dilution

   1:80     1:275  

Literature conformity

   Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.  

RNA consistency

   Consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA003046

  

Antibody HPA011880

  

Antibody HPA058979

  

Antibody CAB017786

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 104, 102.8, 102.3   104, 102.8, 102.3   104, 102.8, 102.3, 87.7, 77.7, 76.8   104, 102.8, 102.3, 27.9  

Antibody dilution

 1:250   1:250   1:80   1:500  

PROTEIN ARRAY
 
 

Antibody HPA003046

  

Antibody HPA011880

  

Antibody HPA058979

  

Antibody CAB017786

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000   1:600    

ANTIGEN INFORMATION
 
 

Antibody HPA003046

  

Antibody HPA011880

  

Antibody HPA058979

  

Antibody CAB017786

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Genetic immunization  

Length (aa)

 104   144   44    

Antigen sequence

 
LAKSSTTVSSFANSKPGSAKKLVIKNFKDKPKLPENYTDETWQKLKEAVE
AIQNSTSIKYNLEELYQAVENLCSYKISANLYKQLRQICEDHIKAQIHQF
REDS
  
GDGNDDEATTSKDGGFSSPSPSAAAAAQEVRSATDGNTSTTPPTSAKKRK
LNSSSSSSSNSSNEREDFDSTSSSSSTPPLQPRDSASPSTSSFCLGVSVA
ASSHVPIQKKLRFEDTLEFVGFDAKMAEESSSSSSSSSPTAATS
  
KHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIM
  

											 
																				

																		

										
											
Matching transcripts

												
																					CUL4B-001 - ENSP00000384109 [100%] 
CUL4B-003 - ENSP00000360373 [100%] 
CUL4B-004 - ENSP00000338919 [100%] 

											 
																						CUL4B-001 - ENSP00000384109 [100%] 
CUL4B-003 - ENSP00000360373 [90%] 
CUL4B-004 - ENSP00000338919 [90%] 

											 
																						CUL4B-001 - ENSP00000384109 [98%] 
CUL4B-003 - ENSP00000360373 [98%] 
CUL4B-004 - ENSP00000338919 [98%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						CUL4A - ENSG00000139842 [100%]

											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CUL4B-001
CUL4B-003
CUL4B-004
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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