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KCNB1

ANTIBODY INFORMATION
 
 

Antibody HPA042434

  

Antibody CAB001979

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Upstate
  

Product name

 HPA042434   06-455  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 13   1  

References

    

Proper citation

 Atlas Antibodies Cat#HPA042434, RRID:AB_2677996    

VALIDATION SUMMARY
 
 

Antibody HPA042434

  

Antibody CAB001979

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Approved    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042434

  

Antibody CAB001979

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic and membranous positivity in neuronal cells.   Immunohistochemical staining of human cerebral cortex shows moderate membranous positivity in neuronal cells.  

Expression

 RNA: detected in 15 tissues
Protein: detected in 10 cell types		   RNA: detected in 15 tissues
Protein: detected in 35 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:750  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042434

  

Antibody CAB001979

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 95.9   95.9  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA042434

  

Antibody CAB001979

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:12000    

ANTIGEN INFORMATION
 
 

Antibody HPA042434

  

Antibody CAB001979

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 115    

Antigen sequence

 
ECATLLDKAVLSPESSIYTTASAKTPPRSPEKHTAIAFNFEAGVHQYIDA
DTDDEGQLLYSVDSSPPKSLPGSTSPKFSTGTRSEKNHFESSPLPTSPKF
LRQNCIYSTEALTGK
  

											 
																				

																		

										
											
Matching transcripts

												
																					KCNB1-001 - ENSP00000360806 [100%] 
KCNB1-002 - ENSP00000489193 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
KCNB1-001
KCNB1-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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