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ANTIBODY INFORMATION
 
 

Antibody HPA036233

  

Antibody HPA036535

  

Antibody CAB001480

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA036233   HPA036535   sc-8983  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 12   12   1  

References

      

Proper citation

 Atlas Antibodies Cat#HPA036233, RRID:AB_2675012   Atlas Antibodies Cat#HPA036535, RRID:AB_2675178   Santa Cruz Biotechnology Cat#sc-8983, RRID:AB_2213986  

VALIDATION SUMMARY
 
 

Antibody HPA036233

  

Antibody HPA036535

  

Antibody CAB001480

  
Immunocytochemistry


Validated
  

Validated
   Supported  
Immunohistochemistry

Supported     Supported  
Western blot


Validated
   Supported   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036233

  

Antibody HPA036535

  

Antibody CAB001480

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA036535.		  
Validated

Antibody staining overlaps with antibody HPA036233.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.  

Antibody dilution

 1:35   1:38   1:62  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036233

  

Antibody HPA036535

  

Antibody CAB001480

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human tonsil shows strong nuclear positivity.     Immunohistochemical staining of human tonsil shows strong positivity.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 79 cell types		     RNA: detected in 37 tissues
Protein: detected in 78 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:25     1:250  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.     Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036233

  

Antibody HPA036535

  

Antibody CAB001480

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		      

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		      

Target mass (kDa)

 33.5, 31      

Loading control

 Total protein image      

Antibody dilution

 1:79      
 

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 33.5, 31   33.5, 31, 24.1   33.5, 31, 24.1, 18.4, 10.7  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA036233

  

Antibody HPA036535

  

Antibody CAB001480

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA036233

  

Antibody HPA036535

  

Antibody CAB001480

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 58   72    

Antigen sequence

 
ELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLE
VVIKNDSN
  
IQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYP
ATQSMTQAVIQGAFTSDDAVDT
  

											 
																				

																		

										
											
Matching transcripts

												
																					USF1-001 - ENSP00000357000 [100%] 
USF1-002 - ENSP00000356999 [100%] 
USF1-003 - ENSP00000356998 [100%] 

											 
																						USF1-001 - ENSP00000357000 [100%] 
USF1-002 - ENSP00000356999 [100%] 
USF1-003 - ENSP00000356998 [100%] 
USF1-011 - ENSP00000435005 [88%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
USF1-001
USF1-002
USF1-003
USF1-011
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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