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CTBP1

ANTIBODY INFORMATION
 
 

Antibody HPA018987

  

Antibody HPA044971

  

Antibody CAB004217

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA018987   HPA044971   sc-17805  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

   CTBP2 - ENSG00000175029 [89%]
    

Released in version

 4   10   2  

References

      

Proper citation

 Atlas Antibodies Cat#HPA018987, RRID:AB_1847307   Atlas Antibodies Cat#HPA044971, RRID:AB_2679161   Santa Cruz Biotechnology Cat#sc-17805, RRID:AB_627312  

VALIDATION SUMMARY
 
 

Antibody HPA018987

  

Antibody HPA044971

  

Antibody CAB004217

  
Immunocytochemistry


Validated

Validated
     Supported  
Immunohistochemistry

Approved   Approved   Approved  
Western blot


Validated
   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA018987

  

Antibody HPA044971

  

Antibody CAB004217

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		      

     

Figure description

 Nuclear region of segmented cells in 10x-images      

Antibody dilution

 1:40      
 

Formal validation: Tagged protein
Validated

Antibody staining overlaps with GFP tagged protein		      

     

Figure description

 Immunofluorescent staining of transgenic HeLa cells show antibody staining in nucleoplasm and GFP expression in nucleoplasm.      

Antibody dilution

 1:40      
 

Standard validation

 Supported     Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.     Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.  

Antibody dilution

 1:38     1:75  

Literature conformity

 The subcellular location is supported by literature.     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA018987

  

Antibody HPA044971

  

Antibody CAB004217

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human testis shows moderate nuclear positivity in a subset of cells in seminiferus ducts.   Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.   Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 49 cell types		   RNA: detected in 37 tissues
Protein: detected in 49 cell types		   RNA: detected in 37 tissues
Protein: detected in 74 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:400   1:80   1:300  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA018987

  

Antibody HPA044971

  

Antibody CAB004217

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		      

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		      

Target mass (kDa)

 47.5, 46.4, 30.9, 19.4      

Loading control

 Total protein image      

Antibody dilution

 1:83      
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 47.5, 46.4, 30.9, 19.4   106.2, 56.1, 48.9, 47.5, 46.4, 21.6, 20.3, 18.6, 15.9   47.5, 46.4, 30.9, 21.6, 20.3, 19.4, 18.6, 18.1, 15.9, 7.5, 2.5  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA018987

  

Antibody HPA044971

  

Antibody CAB004217

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA018987

  

Antibody HPA044971

  

Antibody CAB004217

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 60   28    

Antigen sequence

 
YPPGVVGVAPTGIPAAVEGIVPSAMSLSHGLPPVAHPPHAPSPGQTVKPE
ADRDHASDQL
  
QEIHEKVLNEVVGAMMYHTFSLTREDLE
  

											 
																				

																		

										
											
Matching transcripts

												
																					CTBP1-001 - ENSP00000290921 [100%] 
CTBP1-003 - ENSP00000372411 [100%] 
CTBP1-010 - ENSP00000422364 [100%] 
CTBP1-015 - ENSP00000422148 [100%] 

											 
																						CTBP1-001 - ENSP00000290921 [86%] 
CTBP1-002 - ENSP00000420983 [86%] 
CTBP1-003 - ENSP00000372411 [86%] 
CTBP1-005 - ENSP00000424684 [86%] 
CTBP1-006 - ENSP00000425053 [86%] 
CTBP1-013 - ENSP00000426470 [86%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						CTBP2 - ENSG00000175029 [89%]

											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CTBP1-001
CTBP1-002
CTBP1-003
CTBP1-005
CTBP1-006
CTBP1-010
CTBP1-013
CTBP1-015
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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