Fields »
ABOUT
HELP
BLOG
ADAR

ANTIBODY INFORMATION
 
 

Antibody HPA003890

  

Antibody HPA051519

  

Antibody CAB056157

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA003890   HPA051519   AMAb90535  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 3   13   10  

References

 4      

Proper citation

 Atlas Antibodies Cat#HPA003890, RRID:AB_1078103   Atlas Antibodies Cat#HPA051519, RRID:AB_2681520   Atlas Antibodies Cat#AMAb90535, RRID:AB_2665578  

VALIDATION SUMMARY
 
 

Antibody HPA003890

  

Antibody HPA051519

  

Antibody CAB056157

  
Immunocytochemistry


Validated

Validated
  

Validated
    
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot


Validated
   Uncertain   Supported  
Protein array

Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003890

  

Antibody HPA051519

  

Antibody CAB056157

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		      

     

Figure description

 Nuclear region of segmented cells in 10x-images      

Antibody dilution

 1:88      
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA051519.		  
Validated

Antibody staining overlaps with antibody HPA003890.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.    

Antibody dilution

 1:88   1:135    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003890

  

Antibody HPA051519

  

Antibody CAB056157

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA051519 across 44 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA003890 across 44 cell lines.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells and subset of glial cells.   Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.   Immunohistochemical staining of human kidney shows moderate nuclear positivity .  

Expression

 RNA: detected in 37 tissues
Protein: detected in 66 cell types		   RNA: detected in 37 tissues
Protein: detected in 70 cell types		   RNA: detected in 37 tissues
Protein: detected in 72 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:800   1:1200   1:1000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA003890

  

Antibody HPA051519

  

Antibody CAB056157

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		      

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		      

Target mass (kDa)

 136.1, 132.6, 103.6      

Loading control

 Total protein image      

Antibody dilution

 1:196      
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 136.1, 132.6, 103.6   136.1, 132.6, 103.6   136.1, 132.6, 103.6  

Antibody dilution

 1:250   1:670   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA003890

  

Antibody HPA051519

  

Antibody CAB056157

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:5400    

ANTIGEN INFORMATION
 
 

Antibody HPA003890

  

Antibody HPA051519

  

Antibody CAB056157

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 139   142    

Antigen sequence

 
SDNQPEGMISESLDNLESMMPNKVRKIGELVRYLNTNPVGGLLEYARSHG
FAAEFKLVDQSGPPHEPKFVYQAKVGGRWFPAVCAHSKKQGKQEAADAAL
RVLIGENEKAERMGFTEVTPVTGASLRRTMLLLSRSPEA
  
ALLTHFLQPIYLKSVTLGYLFSQGHLTRAICCRVTRDGSAFEDGLRHPFI
VNHPKVGRVSIYDSKRQSGKTKETSVNWCLADGYDLEILDGTRGTVDGPR
NELSRVSKKNIFLLFKKLCSFRYRRDLLRLSYGEAKKAARDY
  

											 
																				

																		

										
											
Matching transcripts

												
																					ADAR-001 - ENSP00000357459 [100%] 
ADAR-002 - ENSP00000357456 [100%] 
ADAR-007 - ENSP00000431794 [88%] 

											 
																						ADAR-001 - ENSP00000357459 [100%] 
ADAR-002 - ENSP00000357456 [100%] 
ADAR-007 - ENSP00000431794 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ADAR-001
ADAR-002
ADAR-007
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA003890

												
										 
																				
											
											
											
Antibody HPA051519

												
										 
																				
											
											
											
Antibody CAB056157

												
										 
																		

																	

										
											
1.

												
																						MicroRNA editing facilitates immune elimination of HCMV infected cells

	Nachmani D et al

	PLoS Pathog 2014;10(2):e1003963

			
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						ADAR-related activation of adenosine-to-inosine RNA editing during regeneration

	Witman NM et al

	Stem Cells Dev 2013;22(16):2254-67

			
											 
																						
											 
																						
											 
																				

																		

										
											
3.

												
																						Mutations in ADAR1 cause Aicardi-Goutières syndrome associated with a type I interferon signature

	Rice GI et al

	Nat Genet 2012;44(11):1243-8

			
											 
																						
											 
																						
											 
																				

																		

										
											
4.

												
																						A novel immune resistance mechanism of melanoma cells controlled by the ADAR1 enzyme

	Galore-Haskel G et al

	Oncotarget 2015;6(30):28999-9015

			Application: WB				
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.