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ANTIBODY INFORMATION
 
 

Antibody HPA002642

  

Antibody CAB009008

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA002642   sc-10729  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 2   4  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA002642, RRID:AB_1078469   Santa Cruz Biotechnology Cat#sc-10729, RRID:AB_647474  

VALIDATION SUMMARY
 
 

Antibody HPA002642

  

Antibody CAB009008

  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002642

  

Antibody CAB009008

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows distinct cytoplasmic positivity in trophoblastic cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 4 cell types		   RNA: detected in 37 tissues
Protein: detected in 20 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:90  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002642

  

Antibody CAB009008

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 229, 107, 78, 46, 31, 26, 16.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 114.5   114.5, 13.2  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002642

  

Antibody CAB009008

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1000    

ANTIGEN INFORMATION
 
 

Antibody HPA002642

  

Antibody CAB009008

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 129    

Antigen sequence

 
DQPQKEEDLGPAVHHVYELINQGPSSISQGVLELSCPQALEGQQLLYVTR
VTGLNCTTNHPINPKGLELDPEGSLHHQQKREAPSRSSASSGPQILKCPE
AECFRLRCELGPLHQQESQSLQLHFRVWA
  

											 
																				

																		

										
											
Matching transcripts

												
																					ITGA5-001 - ENSP00000293379 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
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RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA002642

												
										 
																				
											
											
											
Antibody CAB009008

												
										 
																		

																	

										
											
1.

												
																						Survivin promotion of melanoma metastasis requires upregulation of α5 integrin

	McKenzie JA et al

	Carcinogenesis 2013;34(9):2137-44

			Application: IHC				
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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