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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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ITGA5
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    TISSUE

  • CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

ITGA5

Gene description

Integrin, alpha 5 (fibronectin receptor, alpha polypeptide)

Protein class

Cancer-related genes
CD markers
Plasma proteins
Predicted membrane proteins

Predicted localization

Membrane

Number of transcripts

2
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Cell line enhanced (HUVEC TERT2, TIME, U-87 MG)
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Selective membranous expression in smooth muscle and trophoblastic cells in placenta.

ANTIBODY IHC RELIABILITY

Data reliability
description

Paired antibodies with high similarity supports the protein profile.

Reliability score

Supported based on 2 antibodies.
HPA002642 , CAB009008
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PROGNOSTIC SUMMARY

Prognostic marker in renal cancer (unfavourable), cervical cancer (unfavourable), liver cancer (unfavourable), lung cancer (unfavourable), head and neck cancer (unfavourable), colorectal cancer (unfavourable) and thyroid cancer (unfavourable).
Renal cancer p<0.001
Cervical cancer p<0.001
Liver cancer p<0.001
Lung cancer p<0.001
Head and neck cancer p<0.001
Colorectal cancer p<0.001
Thyroid cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Head and neck cancer

STAINING SUMMARY

HPA002642
CAB009008

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Cancer tissues were essentially negative. Weak positivity was often observed in tumor stroma.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Several endometrial cancers along with a few skin, urothelial and liver cancer displayed weak to moderate cytoplasmic and membranous positivity. Remaining cancer tissues were negative.

GENE INFORMATION

Gene name

ITGA5 (HGNC Symbol)

Synonyms

CD49e, FNRA

Description

Integrin, alpha 5 (fibronectin receptor, alpha polypeptide) (HGNC Symbol)

Entrez gene summary

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes. [provided by RefSeq, Oct 2015]

Chromosome

12

Cytoband

q13.13

Chromosome location (bp)

54395261 - 54419460

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000161638 (version 83.38)

Entrez gene

3678

UniProt

P08648 (UniProt - Evidence at protein level)

neXtProt

NX_P08648

Antibodypedia

ITGA5 antibodies

PROTEIN BROWSER

ITGA5-001
ITGA5-012

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

ITGA5-001
ENSP00000293379
ENST00000293379
P08648 [Direct mapping]
Integrin alpha-5 Integrin alpha-5 heavy chain Integrin alpha-5 light chain
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CD markers
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001525 [angiogenesis]
GO:0001618 [virus receptor activity]
GO:0001726 [ruffle]
GO:0005154 [epidermal growth factor receptor binding]
GO:0005161 [platelet-derived growth factor receptor binding]
GO:0005178 [integrin binding]
GO:0005515 [protein binding]
GO:0005737 [cytoplasm]
GO:0005783 [endoplasmic reticulum]
GO:0005794 [Golgi apparatus]
GO:0005886 [plasma membrane]
GO:0005911 [cell-cell junction]
GO:0005925 [focal adhesion]
GO:0007044 [cell-substrate junction assembly]
GO:0007155 [cell adhesion]
GO:0007157 [heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules]
GO:0007159 [leukocyte cell-cell adhesion]
GO:0007229 [integrin-mediated signaling pathway]
GO:0007411 [axon guidance]
GO:0007596 [blood coagulation]
GO:0007613 [memory]
GO:0008305 [integrin complex]
GO:0009897 [external side of plasma membrane]
GO:0009986 [cell surface]
GO:0010811 [positive regulation of cell-substrate adhesion]
GO:0030198 [extracellular matrix organization]
GO:0030335 [positive regulation of cell migration]
GO:0030949 [positive regulation of vascular endothelial growth factor receptor signaling pathway]
GO:0031410 [cytoplasmic vesicle]
GO:0031589 [cell-substrate adhesion]
GO:0032587 [ruffle membrane]
GO:0033631 [cell-cell adhesion mediated by integrin]
GO:0034113 [heterotypic cell-cell adhesion]
GO:0035313 [wound healing, spreading of epidermal cells]
GO:0035987 [endodermal cell differentiation]
GO:0043184 [vascular endothelial growth factor receptor 2 binding]
GO:0045202 [synapse]
GO:0046718 [viral entry into host cell]
GO:0046872 [metal ion binding]
GO:0050731 [positive regulation of peptidyl-tyrosine phosphorylation]
GO:0050839 [cell adhesion molecule binding]
GO:0050900 [leukocyte migration]
GO:0071062 [alphav-beta3 integrin-vitronectin complex]
GO:2000811 [negative regulation of anoikis]
Show all
1049 aa
114.5 kDa
Yes 1
ITGA5-012
ENSP00000450267
ENST00000547197
H0YIV7 [Direct mapping]
Integrin alpha-5
Show all
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0016021 [integral component of membrane]
Show all
119 aa
13.2 kDa
No 1

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