PIGR - Antibodies - The Human Protein Atlas

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PIGR

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA006154

Antibody HPA012012

Antibody CAB009454

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA006154

HPA012012

sc-20656

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA006154, RRID:AB_1855370

Atlas Antibodies Cat#HPA012012, RRID:AB_1855371

Santa Cruz Biotechnology Cat#sc-20656, RRID:AB_2164819

VALIDATION SUMMARY

Antibody HPA006154

Antibody HPA012012

Antibody CAB009454

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA006154

Antibody HPA012012

Antibody CAB009454

Standard validation

Uncertain

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleus, nucleoli & cytosol.

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Antibody dilution

1:20

1:31

Literature conformity

The subcellular location is not consistent with literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA006154

Antibody HPA012012

Antibody CAB009454

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Standard validation

Supported

Figure description

Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.

Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 25 tissues
Protein: detected in 39 cell types

RNA: detected in 25 tissues
Protein: detected in 32 cell types

RNA: detected in 25 tissues
Protein: detected in 33 cell types

Retrieval

HIER pH6

Antibody dilution

1:75

1:150

1:350

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA006154

Antibody HPA012012

Antibody CAB009454

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

83.3

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA006154

Antibody HPA012012

Antibody CAB009454

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

ANTIGEN INFORMATION

Antibody HPA006154

Antibody HPA012012

Antibody CAB009454

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

143

129

Antigen sequence

GDTLWRTTVEIKIIEGEPNLKVPGNVTAVLGETLKVPCHFPCKFSSYEKY
WCKWNNTGCQALPSQDEGPSKAFVNCDENSRLVSLTLNLVTRADEGWYWC
GVKQGHFYGETAAVYVAVEERKAAGSRDVSLAKADAAPDEKVL

LLFSVVINQLRLSDAGQYLCQAGDDSNSNKKNADLQVLKPEPELVYEDLR
GSVTFHCALGPEVANVAKFLCRQSSGENCDVVVNTLGKRAPAFEGRILLN
PQDKDGSFSVVITGLRKEDAGRYLCGAHS

Matching transcripts

PIGR-001 - ENSP00000348888 [100%]

Other gene match

ANTIGEN VIEW

PIGR-001

RELEVANT PUBLICATIONS

Antibody HPA006154

Antibody HPA012012

Antibody CAB009454

Age-related expression of the polymeric immunoglobulin receptor (pIgR) in the gastric mucosa of young pigs
Trevisi P et al
PLoS One 2013;8(11):e81473
Application: IHC

Expression and prognostic significance of the polymeric immunoglobulin receptor in epithelial ovarian cancer
Berntsson J et al
J Ovarian Res 2014;7:26

Expression and prognostic significance of the polymeric immunoglobulin receptor in esophageal and gastric adenocarcinoma
Fristedt R et al
J Transl Med 2014;12:83

Reduced expression of the polymeric immunoglobulin receptor in pancreatic and periampullary adenocarcinoma signifies tumour progression and poor prognosis
Fristedt R et al
PLoS One 2014;9(11):e112728
Application: IHC

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.