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ANTIBODY INFORMATION
 
 

Antibody HPA006154

  

Antibody HPA012012

  

Antibody CAB009454

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA006154   HPA012012   sc-20656  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 5   5   3  

References

   4    

Proper citation

 Atlas Antibodies Cat#HPA006154, RRID:AB_1855370   Atlas Antibodies Cat#HPA012012, RRID:AB_1855371   Santa Cruz Biotechnology Cat#sc-20656, RRID:AB_2164819  

VALIDATION SUMMARY
 
 

Antibody HPA006154

  

Antibody HPA012012

  

Antibody CAB009454

  
Immunocytochemistry

Uncertain   Uncertain    
Immunohistochemistry

Supported  

Validated
  

Validated
  
Western blot

Uncertain   Supported   Uncertain  
Protein array

Supported   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA006154

  

Antibody HPA012012

  

Antibody CAB009454

  

Standard validation

 Uncertain   Uncertain    

     

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleus, nucleoli & cytosol.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.    

Antibody dilution

 1:20   1:31    

Literature conformity

 The subcellular location is not consistent with literature.   The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA006154

  

Antibody HPA012012

  

Antibody CAB009454

  

Formal validation: Orthogonal

  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 
 

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 25 tissues
Protein: detected in 39 cell types		   RNA: detected in 25 tissues
Protein: detected in 32 cell types		   RNA: detected in 25 tissues
Protein: detected in 33 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:150   1:350  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA006154

  

Antibody HPA012012

  

Antibody CAB009454

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 83.3   83.3   83.3  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA006154

  

Antibody HPA012012

  

Antibody CAB009454

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA006154

  

Antibody HPA012012

  

Antibody CAB009454

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 143   129    

Antigen sequence

 
GDTLWRTTVEIKIIEGEPNLKVPGNVTAVLGETLKVPCHFPCKFSSYEKY
WCKWNNTGCQALPSQDEGPSKAFVNCDENSRLVSLTLNLVTRADEGWYWC
GVKQGHFYGETAAVYVAVEERKAAGSRDVSLAKADAAPDEKVL
  
LLFSVVINQLRLSDAGQYLCQAGDDSNSNKKNADLQVLKPEPELVYEDLR
GSVTFHCALGPEVANVAKFLCRQSSGENCDVVVNTLGKRAPAFEGRILLN
PQDKDGSFSVVITGLRKEDAGRYLCGAHS
  

											 
																				

																		

										
											
Matching transcripts

												
																					PIGR-001 - ENSP00000348888 [100%] 

											 
																						PIGR-001 - ENSP00000348888 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PIGR-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA006154

												
										 
																				
											
											
											
Antibody HPA012012

												
										 
																				
											
											
											
Antibody CAB009454

												
										 
																		

																	

										
											
1.

												
																					
											 
																							Age-related expression of the polymeric immunoglobulin receptor (pIgR) in the gastric mucosa of young pigs

	Trevisi P et al

	PLoS One 2013;8(11):e81473

			Application: IHC				
											 
																						
											 
																				

																		

										
											
2.

												
																					
											 
																							Expression and prognostic significance of the polymeric immunoglobulin receptor in epithelial ovarian cancer

	Berntsson J et al

	J Ovarian Res 2014;7:26

			
											 
																						
											 
																				

																		

										
											
3.

												
																					
											 
																							Expression and prognostic significance of the polymeric immunoglobulin receptor in esophageal and gastric adenocarcinoma

	Fristedt R et al

	J Transl Med 2014;12:83

			
											 
																						
											 
																				

																		

										
											
4.

												
																					
											 
																							Reduced expression of the polymeric immunoglobulin receptor in pancreatic and periampullary adenocarcinoma signifies tumour progression and poor prognosis

	Fristedt R et al

	PLoS One 2014;9(11):e112728

			Application: IHC				
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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