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Immunofluorescent staining of human cell line A-431 shows localization to nucleoli fibrillar center & cytosol.
Antibody dilution
1:57
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA034986
Standard validation
Approved
Figure description
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 80 cell types
Retrieval
HIER pH6
Antibody dilution
1:500
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA034986
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416634)
Target mass (kDa)
27.7, 23.6
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA034986
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
