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ANTIBODY INFORMATION
 
 

Antibody HPA031024

  

Antibody HPA031025

  

Antibody CAB006262

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Abnova
  

Product name

 HPA031024   HPA031025   H00000213-M01  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 13   13   3  

References

      

Proper citation

 Atlas Antibodies Cat#HPA031024, RRID:AB_2673703   Atlas Antibodies Cat#HPA031025, RRID:AB_2673704   Abnova Corporation Cat#H00000213-M01, RRID:AB_425297  

VALIDATION SUMMARY
 
 

Antibody HPA031024

  

Antibody HPA031025

  

Antibody CAB006262

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA031024

  

Antibody HPA031025

  

Antibody CAB006262

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA031025.		  
Validated

Antibody staining overlaps with antibody HPA031024.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line Hep G2 shows localization to endoplasmic reticulum & the Golgi apparatus.   Immunofluorescent staining of human cell line Hep G2 shows localization to endoplasmic reticulum & the Golgi apparatus.    

Antibody dilution

 1:66   1:22    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA031024

  

Antibody HPA031025

  

Antibody CAB006262

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA031025 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA031024 across 46 cell lines.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human liver shows positivity in plasma.   Immunohistochemical staining of human ovary shows distinct extracellular positivity.   Immunohistochemical staining of human fallopian tube shows distinct positivity in plasma.  

Expression

 RNA: detected in 19 tissues
Protein: detected in 59 cell types		   RNA: detected in 19 tissues
Protein: detected in 58 cell types		   RNA: detected in 19 tissues
Protein: detected in 48 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:6000   1:3000   1:7000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 No internal RNA expression data available for correlation.   No internal RNA expression data available for correlation.   No internal RNA expression data available for correlation.  

WESTERN BLOT
 
 

Antibody HPA031024

  

Antibody HPA031025

  

Antibody CAB006262

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 69.4, 69.2, 56.2, 52.1, 51.5, 47.3   69.4, 69.2, 45.2, 45.1, 22.9   69.4, 69.2, 56.2, 52.1, 51.5, 47.3, 45.2, 45.1, 22.9  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA031024

  

Antibody HPA031025

  

Antibody CAB006262

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA031024

  

Antibody HPA031025

  

Antibody CAB006262

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 78   92    

Antigen sequence

 
ADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRL
AKTYETTLEKCCAAADPHECYAKVFDEF
  
HKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFAKT
CVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQ
  

											 
																				

																		

										
											
Matching transcripts

												
																					ALB-001 - ENSP00000295897 [100%] 
ALB-002 - ENSP00000384695 [100%] 
ALB-009 - ENSP00000401820 [100%] 
ALB-011 - ENSP00000421027 [100%] 
ALB-013 - ENSP00000422784 [100%] 
ALB-016 - ENSP00000426179 [100%] 

											 
																						ALB-001 - ENSP00000295897 [100%] 
ALB-005 - ENSP00000392541 [100%] 
ALB-013 - ENSP00000422784 [100%] 
ALB-201 - ENSP00000483421 [100%] 
ALB-202 - ENSP00000480485 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ALB-001
ALB-002
ALB-005
ALB-009
ALB-011
ALB-013
ALB-016
ALB-201
ALB-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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