POLR2H - Antibodies - The Human Protein Atlas

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POLR2H

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA037745

Antibody HPA055813

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA037745

HPA055813

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA037745, RRID:AB_10672914

VALIDATION SUMMARY

Antibody HPA037745

Antibody HPA055813

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA037745

Antibody HPA055813

Standard validation

Uncertain

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Antibody dilution

1:34

Literature conformity

The subcellular location is not consistent with literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA037745

Antibody HPA055813

Standard validation

Supported

Figure description

Immunohistochemical staining of human stomach shows strong nuclear and cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 72 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA037745

Antibody HPA055813

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

19.8, 17.1, 17, 13.8

17.1, 17, 13

Antibody dilution

1:250

1:170

PROTEIN ARRAY

Antibody HPA037745

Antibody HPA055813

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:1350

ANTIGEN INFORMATION

Antibody HPA037745

Antibody HPA055813

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

AGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVD
LGDKFRLVIASTLYEDGTLDDGEY

PTDDRPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMRLQ
GDANNLHGFEVDSRVYLLMKKLAF

Matching transcripts

POLR2H-001 - ENSP00000392913 [100%]
POLR2H-005 - ENSP00000409065 [100%]
POLR2H-006 - ENSP00000411883 [100%]
POLR2H-009 - ENSP00000415536 [100%]

POLR2H-001 - ENSP00000392913 [100%]
POLR2H-003 - ENSP00000398622 [100%]
POLR2H-008 - ENSP00000399882 [100%]
POLR2H-005 - ENSP00000409065 [99%]

Other gene match

ANTIGEN VIEW

POLR2H-001	POLR2H-003	POLR2H-005	POLR2H-006	POLR2H-008	POLR2H-009

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.