Fields »
ABOUT
HELP
BLOG
ABCE1

ANTIBODY INFORMATION
 
 

Antibody HPA036846

  

Antibody CAB012476

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Abcam plc
  

Product name

 HPA036846   32270  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 13   4  

References

    

Proper citation

 Atlas Antibodies Cat#HPA036846, RRID:AB_2675346    

VALIDATION SUMMARY
 
 

Antibody HPA036846

  

Antibody CAB012476

  
Immunocytochemistry


Validated
    
Immunohistochemistry

  Approved  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036846

  

Antibody CAB012476

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		    

   

Figure description

 Cytoplasmic region of segmented cells in 10x-images    

Antibody dilution

 1:105    
 

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.    

Antibody dilution

 1:105    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036846

  

Antibody CAB012476

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 77 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:300  

Literature conformity

   No avaliable gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036846

  

Antibody CAB012476

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 67.3   67.3, 23.4  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA036846

  

Antibody CAB012476

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:6000    

ANTIGEN INFORMATION
 
 

Antibody HPA036846

  

Antibody CAB012476

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 88    

Antigen sequence

 
KAAKGTVGSILDRKDETKTQAIVCQQLDLTHLKERNVEDLSGGELQRFAC
AVVCIQKADIFMFDEPSSYLDVKQRLKAAITIRSLINP
  

											 
																				

																		

										
											
Matching transcripts

												
																					ABCE1-001 - ENSP00000296577 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ABCE1-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.