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ANTIBODY INFORMATION
 
 

Antibody HPA001384

  

Antibody CAB007773

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Epitomics
  

Product name

 HPA001384   1688-1  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Supernatant  

Other gene match

    

Released in version

 3   3  

References

    

Proper citation

 Atlas Antibodies Cat#HPA001384, RRID:AB_1080144    

VALIDATION SUMMARY
 
 

Antibody HPA001384

  

Antibody CAB007773

  
Immunocytochemistry

Approved    
Immunohistochemistry


Validated
  

Validated
  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001384

  

Antibody CAB007773

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane & cytosol.    

Antibody dilution

 1:189    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001384

  

Antibody CAB007773

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.  
 

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 35 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in the molecular layer.   Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in the molecular layer.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 54 cell types		   RNA: detected in 36 tissues
Protein: detected in 49 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001384

  

Antibody CAB007773

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 72.1, 69.5   72.1, 69.5  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001384

  

Antibody CAB007773

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA001384

  

Antibody CAB007773

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 133    

Antigen sequence

 
GLLRVLTVPCQKIGTQGNVNFGGRPQLPGSHPATWSAGGIISRIKSYSFP
KPGHRKSSPAQGNRQESTVSFNPYEPELAPWAADKGPQREALPMDTEVYE
SPYADPEEIRPKEVYLDRKLLTLEDKELGSGNF
  

											 
																				

																		

										
											
Matching transcripts

												
																					SYK-001 - ENSP00000364907 [100%] 
SYK-002 - ENSP00000364898 [100%] 
SYK-003 - ENSP00000364899 [83%] 
SYK-004 - ENSP00000364904 [83%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
SYK-001
SYK-002
SYK-003
SYK-004
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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