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CDX2

ANTIBODY INFORMATION
 
 

Antibody HPA045669

  

Antibody HPA049580

  

Antibody CAB002221

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Novocastra
  

Product name

 HPA045669   HPA049580   NCL-CDX2  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 12   12   1  

References

      

Proper citation

 Atlas Antibodies Cat#HPA045669, RRID:AB_2679413   Atlas Antibodies Cat#HPA049580, RRID:AB_2680823    

VALIDATION SUMMARY
 
 

Antibody HPA045669

  

Antibody HPA049580

  

Antibody CAB002221

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry

   

Validated
  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA045669

  

Antibody HPA049580

  

Antibody CAB002221

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA049580.		  
Validated

Antibody staining overlaps with antibody HPA045669.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.    

Antibody dilution

 1:28   1:28    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA045669

  

Antibody HPA049580

  

Antibody CAB002221

  

Formal validation: Orthogonal

    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
 

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Orthogonal

    
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

   
 

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

     Supported  

     

Figure description

     Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.  

Expression

     RNA: detected in 6 tissues
Protein: detected in 5 cell types		  

Retrieval

     HIER pH6  

Antibody dilution

     1:50  

Literature conformity

     Consistent with extensive gene/protein characterization data.  

RNA consistency

     Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA045669

  

Antibody HPA049580

  

Antibody CAB002221

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 33.5   33.5   33.5  

Antibody dilution

 1:250   1:140   1:500  

PROTEIN ARRAY
 
 

Antibody HPA045669

  

Antibody HPA049580

  

Antibody CAB002221

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:1100    

ANTIGEN INFORMATION
 
 

Antibody HPA045669

  

Antibody HPA049580

  

Antibody CAB002221

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 46   41    

Antigen sequence

 
MYVSYLLDKDVSMYPSSVRHSGGLNLAPQNFVSPPQYPDYGGYHVA
  
PGPLRSVPEPLSPVSSLQASVPGSVPGVLGPTGGVLNPTVT
  

											 
																				

																		

										
											
Matching transcripts

												
																					CDX2-001 - ENSP00000370408 [100%] 

											 
																						CDX2-001 - ENSP00000370408 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CDX2-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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