TTC7B - Antibodies - The Human Protein Atlas

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TTC7B

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA058363

Antibody HPA059577

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA058363

HPA059577

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA058363, RRID:AB_2683692

Atlas Antibodies Cat#HPA059577, RRID:AB_2684069

VALIDATION SUMMARY

Antibody HPA058363

Antibody HPA059577

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA058363

Antibody HPA059577

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line PC-3 shows localization to plasma membrane.

Antibody dilution

1:87

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA058363

Antibody HPA059577

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.

Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.

Expression

RNA: detected in 37 tissues
Protein: detected in 22 cell types

RNA: detected in 37 tissues
Protein: detected in 28 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

1:85

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA058363

Antibody HPA059577

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

94.2

94.2, 36.2

Antibody dilution

1:280

1:290

PROTEIN ARRAY

Antibody HPA058363

Antibody HPA059577

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3350

1:3500

ANTIGEN INFORMATION

Antibody HPA058363

Antibody HPA059577

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

SECQWERIPELVKQLSAKLIANDDMAELLLGESKLEQYLKEHPLRQGASP
RGPKPQLTEVRKHLTAALDRGNLKSEFLQESNLIMAKLNYVEGDY

HYHDALNIIDMALSEYPENFILLFSKVKLQSLCRGPDEALLTCKHMLQIW
KSCYNLTNPSDSGRGSSLLDRTIADRRQLNTITLPDFSD

Matching transcripts

TTC7B-001 - ENSP00000336127 [100%]

TTC7B-001 - ENSP00000336127 [100%]
TTC7B-015 - ENSP00000451440 [100%]

Other gene match

ANTIGEN VIEW

TTC7B-001	TTC7B-015

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.