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ANTIBODY INFORMATION
 
 

Antibody HPA068845

  

Antibody CAB004619

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA068845   sc-5285  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 14   2  

References

    

Proper citation

 Atlas Antibodies Cat#HPA068845, RRID:AB_2686048   Santa Cruz Biotechnology Cat#sc-5285, RRID:AB_628447  

VALIDATION SUMMARY
 
 

Antibody HPA068845

  

Antibody CAB004619

  
Immunocytochemistry

Supported    
Immunohistochemistry

  Approved  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA068845

  

Antibody CAB004619

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line RH-30 shows localization to nucleoli.    

Antibody dilution

 1:200    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA068845

  

Antibody CAB004619

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human placenta shows strong cytoplasmic and nuclear positivity in cytotrophoblasts.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 41 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:25  

Literature conformity

   Consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA068845

  

Antibody CAB004619

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 71.6, 49.1, 11.4   71.6, 49.1, 24, 18.1, 11.4  

Antibody dilution

 1:700   1:500  

PROTEIN ARRAY
 
 

Antibody HPA068845

  

Antibody CAB004619

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:8400    

ANTIGEN INFORMATION
 
 

Antibody HPA068845

  

Antibody CAB004619

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 81    

Antigen sequence

 
LLKVMIHPDPERRPSAMALVKHSVLLSASRKSAEQLRIELNAEKFKNSLL
QKELKKAQMAKAAAEERALFTDRMATRSTTQ
  

											 
																				

																		

										
											
Matching transcripts

												
																					WEE1-001 - ENSP00000402084 [100%] 
WEE1-002 - ENSP00000299613 [100%] 
WEE1-008 - ENSP00000432284 [95%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
WEE1-001
WEE1-002
WEE1-008
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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