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Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.
Antibody dilution
1:43
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA037422
Antibody HPA037423
Antibody CAB032501
Standard validation
Uncertain
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
Immunohistochemical staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 32 cell types
RNA: detected in 37 tissues Protein: detected in 35 cell types
RNA: detected in 37 tissues Protein: detected in 45 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:250
1:50
1:75
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA037422
Antibody HPA037423
Antibody CAB032501
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411883)
Target mass (kDa)
98.3, 97.4, 69.2
98.3, 97.4
98.3, 97.4, 69.2
Antibody dilution
1:250
1:125
1:500
PROTEIN ARRAY
Antibody HPA037422
Antibody HPA037423
Antibody CAB032501
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
