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SCN11A

ANTIBODY INFORMATION
 
 

Antibody HPA036746

  

Antibody HPA036747

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA036746   HPA036747  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   14  

References

    

Proper citation

 Atlas Antibodies Cat#HPA036746, RRID:AB_10696785   Atlas Antibodies Cat#HPA036747, RRID:AB_2675287  

VALIDATION SUMMARY
 
 

Antibody HPA036746

  

Antibody HPA036747

  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036746

  

Antibody HPA036747

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human rectum shows moderate cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.  

Expression

 RNA: detected in 3 tissues
Protein: detected in 50 cell types		   RNA: detected in 3 tissues
Protein: detected in 52 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:300  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036746

  

Antibody HPA036747

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 204.9, 200.4, 165.7   204.9, 200.4  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA036746

  

Antibody HPA036747

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA036746

  

Antibody HPA036747

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 70   76  

Antigen sequence

 
EERNGNLEGEARKTKVQLALDRFRRAFCFVRHTLEHFCHKWCRKQNLPQQ
KEVAGGCAAQSKDIIPLVME
  
PLKKLYEPIVTTTKRKEEERGAAIIQKAFRKYMMKVTKGDQGDQNDLENG
PHSPLQTLCNGDLSSFGVAKGKVHCD
 

Matching transcripts

 SCN11A-001 - ENSP00000307599 [100%]
SCN11A-002 - ENSP00000408028 [100%]
SCN11A-003 - ENSP00000416757 [100%]
  SCN11A-001 - ENSP00000307599 [100%]
SCN11A-003 - ENSP00000416757 [100%]
 

Other gene match

    
ANTIGEN VIEW
SCN11A-001
SCN11A-002
SCN11A-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.