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POLR3D

ANTIBODY INFORMATION
 
 

Antibody HPA061331

  

Antibody HPA067875

  

Antibody HPA068461

  

Antibody HPA069089

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA061331   HPA067875   HPA068461   HPA069089  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

        

Released in version

 13   14   14   16  

References

        

Proper citation

 Atlas Antibodies Cat#HPA061331, RRID:AB_2684480   Atlas Antibodies Cat#HPA067875, RRID:AB_2685912   Atlas Antibodies Cat#HPA068461, RRID:AB_2685993   Atlas Antibodies Cat#HPA069089, RRID:AB_2686081  

VALIDATION SUMMARY
 
 

Antibody HPA061331

  

Antibody HPA067875

  

Antibody HPA068461

  

Antibody HPA069089

  
Immunocytochemistry


Validated
  

Validated
     Supported  
Immunohistochemistry

 

Validated
  

Validated
    
Western blot

Supported   Supported   Uncertain   Uncertain  
Protein array

Approved   Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA061331

  

Antibody HPA067875

  

Antibody HPA068461

  

Antibody HPA069089

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA067875.		  
Validated

Antibody staining overlaps with antibody HPA061331.		      
 

Standard validation

 Supported   Supported     Supported  

       

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.   Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nuclear speckles.     Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm.  

Antibody dilution

 1:123   1:96     1:60  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA061331

  

Antibody HPA067875

  

Antibody HPA068461

  

Antibody HPA069089

  

Formal validation: Independent

  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 
 
   

Figure description

   Distribution of protein expression (antibody staining).
Spearman correlation with HPA068461 across 72 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA067875 across 72 cell types.		    
 

Standard validation

   Supported   Supported    

       

Figure description

   Immunohistochemical staining of human oral mucosa shows moderate nuclear positivity in squamous epithelial cells.   Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.    

Expression

   RNA: detected in 37 tissues
Protein: detected in 64 cell types		   RNA: detected in 37 tissues
Protein: detected in 57 cell types		    

Retrieval

   HIER pH6   HIER pH6    

Antibody dilution

   1:1100   1:600    

Literature conformity

   Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.    

RNA consistency

   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA061331

  

Antibody HPA067875

  

Antibody HPA068461

  

Antibody HPA069089

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 44.4, 23.4   44.4   44.4, 23.4   44.4, 23.4  

Antibody dilution

 1:410   1:320   1:300   1:200  

PROTEIN ARRAY
 
 

Antibody HPA061331

  

Antibody HPA067875

  

Antibody HPA068461

  

Antibody HPA069089

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:4900   1:3850   1:3600   1:2400  

ANTIGEN INFORMATION
 
 

Antibody HPA061331

  

Antibody HPA067875

  

Antibody HPA068461

  

Antibody HPA069089

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 64   60   64   57  

Antigen sequence

 
DRQREGHGRGRGRPEVIQSHSIFEQGPAEMMKKKGNWDKTVDVSDMGPSH
IINIKKEKRETDEE
  
LPKDVSVAELLRELSLTKEEELLFLQLPDTLPGQPPTQDIKPIKTEVQGE
DGQVVLIKQE
  
DRQREGHGRGRGRPEVIQSHSIFEQGPAEMMKKKGNWDKTVDVSDMGPSH
IINIKKEKRETDEE
  
PRPLLTGARGLIGRRPAPPLTPGRLPSIRSRDLTLGGVKKKTFTPNIISR
KIKEEPK
 

Matching transcripts

 POLR3D-001 - ENSP00000380904 [100%]
POLR3D-003 - ENSP00000303088 [100%]
POLR3D-005 - ENSP00000429677 [100%]
  POLR3D-001 - ENSP00000380904 [100%]
POLR3D-003 - ENSP00000303088 [100%]
  POLR3D-001 - ENSP00000380904 [100%]
POLR3D-003 - ENSP00000303088 [100%]
POLR3D-005 - ENSP00000429677 [100%]
  POLR3D-001 - ENSP00000380904 [100%]
POLR3D-003 - ENSP00000303088 [100%]
POLR3D-005 - ENSP00000429677 [100%]
 

Other gene match

        
ANTIGEN VIEW
POLR3D-001
POLR3D-003
POLR3D-005
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.