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DCXR

ANTIBODY INFORMATION
 
 

Antibody HPA023371

  

Antibody HPA023863

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA023371   HPA023863  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   5  

References

    

Proper citation

 Atlas Antibodies Cat#HPA023371, RRID:AB_1847530   Atlas Antibodies Cat#HPA023863, RRID:AB_1847531  

VALIDATION SUMMARY
 
 

Antibody HPA023371

  

Antibody HPA023863

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry


Validated
   Supported  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA023371

  

Antibody HPA023863

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.   Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.  

Antibody dilution

 1:100   1:7  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA023371

  

Antibody HPA023863

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

   

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human liver shows strong cytoplasmic and dot like nucleolar positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 51 cell types		   RNA: detected in 37 tissues
Protein: detected in 51 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:750   1:75  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA023371

  

Antibody HPA023863

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 25.9, 24, 9.2   25.9, 24, 23.5  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA023371

  

Antibody HPA023863

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA023371

  

Antibody HPA023863

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 68   73  

Antigen sequence

 
IRVNAVNPTVVMTSMGQATWSDPHKAKTMLNRIPLGKFAEVEHVVNAILF
LLSDRSGMTTGSTLPVEG
  
TVQALHATGARVVAVSRTQADLDSLVRECPGIEPVCVDLGDWEATERALG
SVGPVDLLVNNAAVALLQPFLEV
 

Matching transcripts

 DCXR-001 - ENSP00000303356 [100%]
DCXR-021 - ENSP00000464332 [96%]
DCXR-007 - ENSP00000462272 [81%]
  DCXR-001 - ENSP00000303356 [100%]
DCXR-007 - ENSP00000462272 [100%]
DCXR-011 - ENSP00000464507 [100%]
 

Other gene match

    
ANTIGEN VIEW
DCXR-001
DCXR-007
DCXR-011
DCXR-021
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.