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FAM153A

ANTIBODY INFORMATION
 
 

Antibody HPA042585

  

Antibody HPA044022

  

Antibody HPA046691

  

Antibody HPA052337

  

Antibody HPA055498

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042585   HPA044022   HPA046691   HPA052337   HPA055498  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 FAM153B - ENSG00000182230 [100%]
FAM153C - ENSG00000204677 [100%]
   FAM153B - ENSG00000182230 [100%]
   FAM153B - ENSG00000182230 [100%]
FAM153C - ENSG00000204677 [95%]
   FAM153B - ENSG00000182230 [100%]
FAM153C - ENSG00000204677 [100%]
   FAM153B - ENSG00000182230 [100%]
  

Released in version

 13   13   13   13   13  

References

          

Proper citation

 Atlas Antibodies Cat#HPA042585, RRID:AB_2678063   Atlas Antibodies Cat#HPA044022, RRID:AB_2678778   Atlas Antibodies Cat#HPA046691, RRID:AB_2679754   Atlas Antibodies Cat#HPA052337, RRID:AB_2681792   Atlas Antibodies Cat#HPA055498, RRID:AB_2682836  

VALIDATION SUMMARY
 
 

Antibody HPA042585

  

Antibody HPA044022

  

Antibody HPA046691

  

Antibody HPA052337

  

Antibody HPA055498

  
Immunohistochemistry

Uncertain   Uncertain   Uncertain   Uncertain   Uncertain  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Approved   Supported   Supported   Approved   Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042585

  

Antibody HPA044022

  

Antibody HPA046691

  

Antibody HPA052337

  

Antibody HPA055498

  

Standard validation

 Uncertain   Uncertain   Uncertain   Uncertain   Uncertain  

         

Figure description

 Immunohistochemical staining of human heart muscle shows strong cytoplasmic and nuclear positivity in myocytes.   Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in testis.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine pancreas.   Immunohistochemical staining of human kidney shows moderate cytoplasmic and nuclear positivity in renal tubules.   Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.  

Expression

 RNA: detected in 4 tissues
Protein: detected in 70 cell types		   RNA: detected in 4 tissues
Protein: detected in 60 cell types		   RNA: detected in 4 tissues
Protein: detected in 43 cell types		   RNA: detected in 4 tissues
Protein: detected in 50 cell types		   RNA: detected in 4 tissues
Protein: detected in 20 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:1500   1:50   1:3000   1:600   1:175  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.   Not consistent with RNA expression data.   Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042585

  

Antibody HPA044022

  

Antibody HPA046691

  

Antibody HPA052337

  

Antibody HPA055498

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

         

Figure description

          

Target mass (kDa)

 43.7, 43.6, 34.7, 19.8, 16.6, 14.7   43.7, 43.6, 34.7, 14.9, 13.7   43.7, 43.6, 34.7, 20.2, 19.8, 16.6, 14.7, 12.9   43.7, 43.6, 34.7, 20.2, 19.8, 16.6, 14.7, 13.7, 12.9, 4.9   43.7, 43.6, 34.7  

Antibody dilution

 1:250   1:250   1:980   1:1000   1:390  

PROTEIN ARRAY
 
 

Antibody HPA042585

  

Antibody HPA044022

  

Antibody HPA046691

  

Antibody HPA052337

  

Antibody HPA055498

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:30000   1:6000   1:15000   1:500   1:15000  

ANTIGEN INFORMATION
 
 

Antibody HPA042585

  

Antibody HPA044022

  

Antibody HPA046691

  

Antibody HPA052337

  

Antibody HPA055498

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 47   56   37   36   38  

Antigen sequence

 
EDSTITGSHQQMSASPSSAPAEEATEKTKVEEEVKTRKPKKKTRKPS
  
RDEGSLGKPLCPPEILSETLPGSVKKRVCFPSEDHLEEFIAEHLPEASNQ
SLLTVA
  
QRGDLEDLEEHVPGQTVSEEATGVHMMQVDPATPAKS
  
MVDKDTERDIEMKRQLRRLRELHLYSTWKKYQEAMK
  
DPDTLAELLIRDVLQELSSYNGEEEDPEEVKTSLGVPQ
 

Matching transcripts

 FAM153A-002 - ENSP00000422174 [100%]
FAM153A-004 - ENSP00000426689 [100%]
FAM153A-006 - ENSP00000421623 [100%]
FAM153A-022 - ENSP00000411506 [100%]
FAM153A-202 - ENSP00000481950 [100%]
  FAM153A-002 - ENSP00000422174 [100%]
FAM153A-022 - ENSP00000411506 [100%]
FAM153A-202 - ENSP00000481950 [100%]
FAM153A-010 - ENSP00000423766 [80%]
  FAM153A-002 - ENSP00000422174 [97%]
FAM153A-022 - ENSP00000411506 [97%]
FAM153A-202 - ENSP00000481950 [97%]
FAM153A-004 - ENSP00000426689 [95%]
FAM153A-201 - ENSP00000377153 [95%]
FAM153A-006 - ENSP00000421623 [92%]
  FAM153A-002 - ENSP00000422174 [100%]
FAM153A-004 - ENSP00000426689 [100%]
FAM153A-006 - ENSP00000421623 [100%]
FAM153A-010 - ENSP00000423766 [100%]
FAM153A-022 - ENSP00000411506 [100%]
FAM153A-201 - ENSP00000377153 [100%]
FAM153A-202 - ENSP00000481950 [100%]
  FAM153A-002 - ENSP00000422174 [97%]
FAM153A-022 - ENSP00000411506 [97%]
FAM153A-202 - ENSP00000481950 [97%]
 

Other gene match

 FAM153B - ENSG00000182230 [100%]
FAM153C - ENSG00000204677 [100%]
   FAM153B - ENSG00000182230 [100%]
   FAM153B - ENSG00000182230 [100%]
FAM153C - ENSG00000204677 [95%]
   FAM153B - ENSG00000182230 [100%]
FAM153C - ENSG00000204677 [100%]
   FAM153B - ENSG00000182230 [100%]
  
ANTIGEN VIEW
FAM153A-002
FAM153A-004
FAM153A-006
FAM153A-010
FAM153A-022
FAM153A-201
FAM153A-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.