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FAM153C

ANTIBODY INFORMATION
 
 

Antibody HPA042585

  

Antibody HPA046691

  

Antibody HPA052337

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042585   HPA046691   HPA052337  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 FAM153A - ENSG00000170074 [100%]
FAM153B - ENSG00000182230 [100%]
   FAM153A - ENSG00000170074 [97%]
FAM153B - ENSG00000182230 [100%]
   FAM153A - ENSG00000170074 [100%]
FAM153B - ENSG00000182230 [100%]
  

Released in version

 13   13   13  

References

      

Proper citation

 Atlas Antibodies Cat#HPA042585, RRID:AB_2678063   Atlas Antibodies Cat#HPA046691, RRID:AB_2679754   Atlas Antibodies Cat#HPA052337, RRID:AB_2681792  

VALIDATION SUMMARY
 
 

Antibody HPA042585

  

Antibody HPA046691

  

Antibody HPA052337

  
Immunohistochemistry

Uncertain   Uncertain   Uncertain  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Approved   Supported   Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042585

  

Antibody HPA046691

  

Antibody HPA052337

  

Standard validation

 Uncertain   Uncertain   Uncertain  

     

Figure description

 Immunohistochemical staining of human heart muscle shows strong cytoplasmic and nuclear positivity in myocytes.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine pancreas.   Immunohistochemical staining of human kidney shows moderate cytoplasmic and nuclear positivity in renal tubules.  

Expression

 RNA: detected in 12 tissues
Protein: detected in 70 cell types		   RNA: detected in 12 tissues
Protein: detected in 43 cell types		   RNA: detected in 12 tissues
Protein: detected in 50 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:1500   1:3000   1:600  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042585

  

Antibody HPA046691

  

Antibody HPA052337

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 43.7, 43.6, 34.7, 19.8, 16.6, 14.7   43.7, 43.6, 34.7, 20.2, 19.8, 16.6, 14.7, 12.9   43.7, 43.6, 34.7, 20.2, 19.8, 16.6, 14.7, 13.7, 12.9, 4.9  

Antibody dilution

 1:250   1:980   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA042585

  

Antibody HPA046691

  

Antibody HPA052337

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:30000   1:15000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA042585

  

Antibody HPA046691

  

Antibody HPA052337

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 47   37   36  

Antigen sequence

 
EDSTITGSHQQMSASPSSAPAEEATEKTKVEEEVKTRKPKKKTRKPS
  
QRGDLEDLEEHVPGQTVSEEATGVHMMQVDPATPAKS
  
MVDKDTERDIEMKRQLRRLRELHLYSTWKKYQEAMK
 

Matching transcripts

 FAM153C-002 - ENSP00000424623 [100%]
FAM153C-005 - ENSP00000426621 [100%]
FAM153C-201 - ENSP00000481919 [100%]
  FAM153C-001 - ENSP00000381177 [95%]
FAM153C-002 - ENSP00000424623 [95%]
FAM153C-004 - ENSP00000422293 [95%]
FAM153C-005 - ENSP00000426621 [95%]
FAM153C-201 - ENSP00000481919 [95%]
  FAM153C-001 - ENSP00000381177 [100%]
FAM153C-002 - ENSP00000424623 [100%]
FAM153C-004 - ENSP00000422293 [100%]
FAM153C-005 - ENSP00000426621 [100%]
FAM153C-012 - ENSP00000423403 [100%]
FAM153C-201 - ENSP00000481919 [100%]
 

Other gene match

 FAM153A - ENSG00000170074 [100%]
FAM153B - ENSG00000182230 [100%]
   FAM153A - ENSG00000170074 [97%]
FAM153B - ENSG00000182230 [100%]
   FAM153A - ENSG00000170074 [100%]
FAM153B - ENSG00000182230 [100%]
  
ANTIGEN VIEW
FAM153C-001
FAM153C-002
FAM153C-004
FAM153C-005
FAM153C-012
FAM153C-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.