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Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
Antibody dilution
1:75
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA045307
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
Expression
RNA: detected in 37 tissues Protein: detected in 51 cell types
Retrieval
HIER pH6
Antibody dilution
1:50
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA045307
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY414753)
Target mass (kDa)
27.2, 23.4, 20.3, 12
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA045307
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
