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PGAM1

ANTIBODY INFORMATION
 
 

Antibody HPA042528

  

Antibody HPA049237

  

Antibody HPA060483

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042528   HPA049237   HPA060483  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 PGAM4 - ENSG00000226784 [100%]
   PGAM2 - ENSG00000164708 [91%]
PGAM4 - ENSG00000226784 [94%]
   PGAM4 - ENSG00000226784 [100%]
  

Released in version

 13   13   16  

References

      

Proper citation

 Atlas Antibodies Cat#HPA042528, RRID:AB_2678039   Atlas Antibodies Cat#HPA049237, RRID:AB_2680687   Atlas Antibodies Cat#HPA060483, RRID:AB_2684292  

VALIDATION SUMMARY
 
 

Antibody HPA042528

  

Antibody HPA049237

  

Antibody HPA060483

  
Immunocytochemistry

Approved     Approved  
Immunohistochemistry

Approved   Approved    
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA042528

  

Antibody HPA049237

  

Antibody HPA060483

  

Standard validation

 Approved     Approved  

     

Figure description

 Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.     Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.  

Antibody dilution

 1:26     1:47  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.     The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042528

  

Antibody HPA049237

  

Antibody HPA060483

  

Standard validation

 Approved   Approved    

     

Figure description

 Immunohistochemical staining of human adrenal gland shows moderate positivity in glandular cells.   Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts and Leydig cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 49 cell types		   RNA: detected in 37 tissues
Protein: detected in 70 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:200   1:500    

Literature conformity

 No avaliable gene/protein characterization data.   Partly consistent with gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA042528

  

Antibody HPA049237

  

Antibody HPA060483

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 28.8   28.8   28.8  

Antibody dilution

 1:250   1:80   1:160  

PROTEIN ARRAY
 
 

Antibody HPA042528

  

Antibody HPA049237

  

Antibody HPA060483

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:20000   1:500   1:1900  

ANTIGEN INFORMATION
 
 

Antibody HPA042528

  

Antibody HPA049237

  

Antibody HPA060483

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 29   33   25  

Antigen sequence

 
PPMEPDHPFYSNISKDRRYADLTEDQLPS
  
KNLKPIKPMQFLGDEETVRKAMEAVAAQGKAKK
  
YDADLSPAGHEEAKRGGQALRDAGY
 

Matching transcripts

 PGAM1-001 - ENSP00000359991 [100%]
  PGAM1-001 - ENSP00000359991 [100%]
  PGAM1-001 - ENSP00000359991 [100%]
 

Other gene match

 PGAM4 - ENSG00000226784 [100%]
   PGAM2 - ENSG00000164708 [91%]
PGAM4 - ENSG00000226784 [94%]
   PGAM4 - ENSG00000226784 [100%]
  
ANTIGEN VIEW
PGAM1-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.