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Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA031186 across 75 cell types. Spearman correlation with HPA031185 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA031186 across 74 cell types. Spearman correlation with HPA031184 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA031185 across 74 cell types. Spearman correlation with HPA031184 across 75 cell types.
Standard validation
Supported
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human colon shows strong cytoplasmic, membranous and nuclear positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic, membranous and nuclear positivity in glandular cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic, membranous and nuclear positivity in glandular cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 19 tissues Protein: detected in 10 cell types
RNA: detected in 19 tissues Protein: detected in 8 cell types
RNA: detected in 19 tissues Protein: detected in 13 cell types
RNA: detected in 19 tissues Protein: detected in 17 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:150
1:25
1:900
1:900
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA031184
Antibody HPA031185
Antibody HPA031186
Antibody CAB025653
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
35.9, 24.1
35.9
35.9, 24.1
35.9, 24.1, 3.4
Antibody dilution
1:250
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA031184
Antibody HPA031185
Antibody HPA031186
Antibody CAB025653
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.