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CTPS1

ANTIBODY INFORMATION
 
 

Antibody HPA051322

  

Antibody CAB017111

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Origene
  

Product name

 HPA051322   2337  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 12   4  

References

    

Proper citation

 Atlas Antibodies Cat#HPA051322, RRID:AB_2681441    

VALIDATION SUMMARY
 
 

Antibody HPA051322

  

Antibody CAB017111

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

  Approved  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA051322

  

Antibody CAB017111

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & actin filaments.   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.  

Antibody dilution

 1:27   1:75  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051322

  

Antibody CAB017111

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 78 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:300  

Literature conformity

   Partly consistent with gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA051322

  

Antibody CAB017111

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 66.7   66.7  

Antibody dilution

 1:130   1:500  

PROTEIN ARRAY
 
 

Antibody HPA051322

  

Antibody CAB017111

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1100    

ANTIGEN INFORMATION
 
 

Antibody HPA051322

  

Antibody CAB017111

  

Antigen

 Recombinant protein fragment   Genetic immunization  

Length (aa)

 53    

Antigen sequence

 
PYFGLLLASVGRLSHYLQKGCRLSPRDTYSDRSGSSSPDSEITELKFPSI
NHD
  

											 
																				

																		

										
											
Matching transcripts

												
																					CTPS1-001 - ENSP00000361704 [100%] 
CTPS1-002 - ENSP00000361699 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CTPS1-001
CTPS1-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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