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COL6A5

ANTIBODY INFORMATION
 
 

Antibody HPA043138

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA043138  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 8  

References

  

Proper citation

 Atlas Antibodies Cat#HPA043138, RRID:AB_10796687  

VALIDATION SUMMARY
 
 

Antibody HPA043138

  
Immunohistochemistry

Uncertain  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA043138

  

Standard validation

 Uncertain  

 

Figure description

 Immunohistochemical staining of human skin shows moderate positivity in hair follicle and a subset of kerationocytes.  

Expression

 RNA: detected in 2 tissues
Protein: detected in 26 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:75  

Literature conformity

 Partly consistent with gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA043138

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

 

Figure description

  

Target mass (kDa)

 289.5, 88.6  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA043138

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA043138

  

Antigen

 Recombinant protein fragment  

Length (aa)

 101  

Antigen sequence

 
MDVVFLIDNSRNIAKDEFKAVKALVSSVIDNFNIASDPLISDSGDRIALL
SYSPWESSRRKMGTVKTEFDFITYDNQLLMKNHIQTSFQQLNGEATIGRA
L
 

Matching transcripts

 COL6A5-003 - ENSP00000422898 [100%]
COL6A5-201 - ENSP00000265379 [100%]
 

Other gene match

  
ANTIGEN VIEW
COL6A5-003
COL6A5-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.