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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & intermediate filaments.
Antibody dilution
1:48
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA006299
Antibody CAB037321
Standard validation
Supported
Figure description
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in keratinocytes.
Expression
RNA: detected in 6 tissues Protein: detected in 6 cell types
Retrieval
HIER pH6
Antibody dilution
1:75
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA006299
Antibody CAB037321
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
65.4
65.4
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA006299
Antibody CAB037321
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
